南方科技大学知识苑(SUSTech KC): DKK1-targeting cholesterol-modified siRNA implication in hair growth regulation

题名	DKK1-targeting cholesterol-modified siRNA implication in hair growth regulation
作者	Papukashvili，Dimitri; Liu，Cong; Rcheulishvili，Nino; Xie，Fengfei; Wang，Xingyun; Feng，Shunping; Sun，Xiu; Zhang，Chi; Li，Yingyu; He，Yunjiao; Wang，Peng George
通讯作者	He，Yunjiao; Wang，Peng George
发表日期	2023-08-06
DOI	10.1016/j.bbrc.2023.05.047
发表期刊	Biochemical and Biophysical Research Communications 影响因子和分区
ISSN	0006-291X
EISSN	1090-2104
卷号	668页码:55-61
摘要	Despite advancements in medical research, androgenetic alopecia (AGA) remains a humankind problem that still needs to be overcome. To date, clinical practice lacks an ideal treatment for AGA. The Wnt/β-catenin signaling pathway is evidenced to play a key role in hair regrowth, hence, modulating this signaling pathway for AGA therapy appears to be rational. One of the major inhibitors of the canonical Wnt/β-catenin signaling pathway is dickkopf-related protein 1 (DKK1). In this report, we have selected a small interfering RNA (siRNA) targeting DKK1 in vitro via qPCR and then tested its efficacy in vivo on the depilated dorsal skin of the mice. The changes in hair growth in different groups were observed over time. Moreover, the visual observation of the hair growth and hematoxylin and eosin (HE) staining showed that DKK1-targeting siRNA reveals non-inferior results compared with the mice treated with the Food and Drug Administration (FDA)-approved, commercially available minoxidil (5%) topical solution that was used as a positive control. Both– positive control and DKK1-targeting siRNA groups demonstrated significantly superior results compared with the control group that received negative control siRNA. Consequently, siRNAs targeting DKK1 may promote hair growth regulation in the AGA population via potentially activating the Wnt/β-catenin signaling pathway.
关键词	Alopecia DKK1 Hair siRNA Wnt/β-catenin signaling
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Department of Education of Guangdong Province Research Project[2020KZDZX1181] ; Shenzhen Science and Technology Program[KQTD20200909113758004]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:001010977700001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
ESI学科分类	BIOLOGY & BIOCHEMISTRY
Scopus记录号	2-s2.0-85160262841
来源库	Scopus
引用统计	被引频次[WOS]：4
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/536418
专题	南方科技大学医学院_药理学系南方科技大学医学院
作者单位	Department of Pharmacology,School of Medicine,Southern University of Science and Technology,Shenzhen,518000,China
第一作者单位	药理学系; 南方科技大学医学院
通讯作者单位	药理学系; 南方科技大学医学院
第一作者的第一单位	药理学系; 南方科技大学医学院
推荐引用方式 GB/T 7714	Papukashvili，Dimitri,Liu，Cong,Rcheulishvili，Nino,et al. DKK1-targeting cholesterol-modified siRNA implication in hair growth regulation[J]. Biochemical and Biophysical Research Communications,2023,668:55-61.
APA	Papukashvili，Dimitri.,Liu，Cong.,Rcheulishvili，Nino.,Xie，Fengfei.,Wang，Xingyun.,...&Wang，Peng George.(2023).DKK1-targeting cholesterol-modified siRNA implication in hair growth regulation.Biochemical and Biophysical Research Communications,668,55-61.
MLA	Papukashvili，Dimitri,et al."DKK1-targeting cholesterol-modified siRNA implication in hair growth regulation".Biochemical and Biophysical Research Communications 668(2023):55-61.