Early growth response factor 1 upregulates pro-fibrotic genes through activation of TGF-β1/Smad pathway via transcriptional regulation of PAR1 in high-glucose treated HK-2 cells

Xu，Ping1; Zhan，Hui2; Zhang，Rui1; Xu，Xiu jun3; Zhang，Ying1; Le，Ying1; Bi，Jian gang4

2023-07-15

10.1016/j.mce.2023.111953

Molecular and Cellular Endocrinology   影响因子和分区

0303-7207

1872-8057

Tubulointerstitial fibrosis (TIF) makes a key role in diabetic kidney disease (DKD). In this study, we revealed that the expressions of Egr1 and protease-activated receptor 1 (PAR1) were increased in renal tissues of DKD rats. In vitro experiments demonstrated that both Egr1 overexpression and high glucose (HG) condition could promote the expressions of PAR1, fibronectin (FN) and collagen I (COL I). Furthermore, HG stimulation enhanced the binding capacity of Egr1 to PAR1 promoter. Both HG condition and Egr1 upregulation could increase, and thrombin inhibitor did not affect activity of TGF-β1/Smad pathway via PAR1. Collectively, Egr1 is involved in TIF of DKD partly through activating TGF-β1/Smad pathway via transcriptional regulation of PAR1 in HG treated HK-2 cells.

SCI

英语

第一

Guangdong Medical Science and Technology Research Foundation[A2021275]

Cell Biology ; Endocrinology & Metabolism

Cell Biology ; Endocrinology & Metabolism

WOS:001007175600001

ELSEVIER IRELAND LTD

BIOLOGY & BIOCHEMISTRY

2-s2.0-85159621226

Scopus

1.Department of Endocrinology and Metabolism,Shenzhen People's Hospital,The second Clinical Medical College of Jinan University,The First Affiated Hospital,Southern University of Science and Technology,Shenzhen,Guangdong,518020,China
2.Department of Pharmacy,Shenzhen People's Hospital,The second Clinical Medical College of Jinan University,The First Affiated Hospital,Southern University of Science and Technology,Shenzhen,Guangdong,518020,China
3.Shenzhen Municipal Health Commission Office,Shenzhen,Guangdong,518020,China
4.Department of Hepatobiliary Surgery,Shenzhen People's Hospital,The Second Clinical Medical College of Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,Guangdong,518020,China

Xu，Ping,Zhan，Hui,Zhang，Rui,et al. Early growth response factor 1 upregulates pro-fibrotic genes through activation of TGF-β1/Smad pathway via transcriptional regulation of PAR1 in high-glucose treated HK-2 cells[J]. Molecular and Cellular Endocrinology,2023,572.

Xu，Ping.,Zhan，Hui.,Zhang，Rui.,Xu，Xiu jun.,Zhang，Ying.,...&Bi，Jian gang.(2023).Early growth response factor 1 upregulates pro-fibrotic genes through activation of TGF-β1/Smad pathway via transcriptional regulation of PAR1 in high-glucose treated HK-2 cells.Molecular and Cellular Endocrinology,572.

Xu，Ping,et al."Early growth response factor 1 upregulates pro-fibrotic genes through activation of TGF-β1/Smad pathway via transcriptional regulation of PAR1 in high-glucose treated HK-2 cells".Molecular and Cellular Endocrinology 572(2023).