Impacts of residual 3D printing metal powders on immunological response and bone regeneration: an in vivo study

Tang，Jincheng1; Sang，Zhuo2; Zhang，Xiaolei2; Song，Changhui3; Tang，Wei4; Luo，Xiaoping5; Yan，Ming1,6

2023-06-01

10.1007/s10856-023-06727-1

Journal of Materials Science: Materials in Medicine   影响因子和分区

0957-4530

1573-4838

Residual powder is a defect in powder bed fusion-based additive manufacturing (3D printing), and it is difficult to completely remove it from as-printed materials. In addition, it is not necessary to apply 3D printed implants with residual powder in the clinic. The immunological response triggered by the residual powder is an important area of study in medical research. To further understand the possible immunological reactions and hidden dangers caused by residual powders in vivo, this study compared the immunological reactions and osteolysis caused by typical powders for four implant materials: 316 L stainless steel, CoCrMo, CP-Ti, and Ti-6Al-4V (particle size range of 15–45 μm), in a mouse skull model. Furthermore, the possible immunological responses and bone regeneration induced by the four 3D printed implants with residual powder in a rat femur model were compared. In the mouse skull model, it was found that the 316L-S, CoCrMo-S, and especially the 316L-M powders, upregulated the expression of pro-inflammatory factors, increased the ratio of RANKL/OPG, and activated more functional osteoclasts, resulting in more severe bone resorption compared with those in other groups. In the rat femur model, which is more suitable for clinical practice, there is no bone resorption in implants with residual powders, but they show good bone regeneration and integration ability because of their original roughness. The results indicate that the expressions of inflammatory cytokines in all experimental groups were the same as those in the control group, showing good biological safety. The results answered some critical questions related to additively manufactured medical materials in vivo and indicated that as-printed implants may have great potential in future clinical applications. Graphical Abstract: [Figure not available: see fulltext.]

Bone regeneration Immunological response Metal powder Osteolysis Powder bed fusion additive manufacturing

SCI ; EI

英语

第一 ; 通讯

Basic and Applied Basic Research Foundation of Guangdong Province[2020B1515120013];National Natural Science Foundation of China[51971108];

Engineering ; Materials Science

Engineering, Biomedical ; Materials Science, Biomaterials

WOS:000995183500001

SPRINGER

20232214155661

Additives ; Aluminum alloys ; Bone ; Chromium alloys ; Cobalt alloys ; Metal implants ; Molybdenum alloys ; Particle size ; Powder metals ; Rats ; Ternary alloys ; Titanium alloys ; Vanadium alloys

Biological Materials and Tissue Engineering:461.2 ; Prosthetics:462.4 ; Aluminum Alloys:541.2 ; Titanium and Alloys:542.3 ; Chromium and Alloys:543.1 ; Molybdenum and Alloys:543.3 ; Vanadium and Alloys:543.6 ; Nonferrous Metals and Alloys excluding Alkali and Alkaline Earth Metals:549.3 ; Printing Equipment:745.1.1 ; Chemical Agents and Basic Industrial Chemicals:803

MATERIALS SCIENCE

2-s2.0-85160202666

Scopus

1.Department of Materials Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.The Eighth Affiliated Hospital,Sun Yat- sen University,Shenzhen,518033,China
3.Department of Mechanical and Automotive Engineering,South China University of Technology,Guangzhou,510641,China
4.Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China
5.Nanjing Stomatological Hospital Medical School of Nanjing University,Nanjing,210008,China
6.Jiaxing Research Institute,Southern University of Science and Technology,Jiaxing,314001,China

Tang，Jincheng,Sang，Zhuo,Zhang，Xiaolei,et al. Impacts of residual 3D printing metal powders on immunological response and bone regeneration: an in vivo study[J]. Journal of Materials Science: Materials in Medicine,2023,34(6).

Tang，Jincheng.,Sang，Zhuo.,Zhang，Xiaolei.,Song，Changhui.,Tang，Wei.,...&Yan，Ming.(2023).Impacts of residual 3D printing metal powders on immunological response and bone regeneration: an in vivo study.Journal of Materials Science: Materials in Medicine,34(6).

Tang，Jincheng,et al."Impacts of residual 3D printing metal powders on immunological response and bone regeneration: an in vivo study".Journal of Materials Science: Materials in Medicine 34.6(2023).