南方科技大学知识苑(SUSTech KC): Simultaneous Multisite Extracellular Electrophysiological Recordings During Electrical Stimulation in Anesthetized Rats

题名	Simultaneous Multisite Extracellular Electrophysiological Recordings During Electrical Stimulation in Anesthetized Rats
作者	Lin，Muxin 1; Wang，Ying2 ; Ping，Yong 1; Chen，Fujun 1
发表日期	2023-05-01
DOI	10.1002/cpz1.787
发表期刊	Current protocols 影响因子和分区
EISSN	2691-1299
卷号	3 期号:5
摘要	Analyzing neuronal activities is essential to deciphering the function of neural circuits. In anesthetized rodents, simultaneous multisite recording of extracellular electrophysiological activity with defined electrical stimulation is a powerful tool to dissect reciprocal relationships between brain structures. Here, we present a protocol to simultaneously record from the subthalamic nucleus and substantia nigra pars reticulata while stimulating the pedunculopontine tegmental nucleus in anesthetized rats. This protocol describes the preparation of recording and stimulating electrodes, surgery setup, and detailed recording techniques. Basic post-recording data analysis methods are included as well. This protocol can be adapted to other brain areas of interest following the outlined procedures. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Assembly of recording and stimulating electrodes Basic Protocol 2: Implantation of recording and stimulating electrodes in an anesthetized rat Basic Protocol 3: Simultaneous recording from the STN and SNr with PPTg stimulation Basic Protocol 4: Histological verification of recording sites Basic Protocol 5: Analysis of electrophysiological data.
关键词	electrical stimulation electrophysiology neural circuits
相关链接	[Scopus记录]
语种	英语
学校署名	其他
Scopus记录号	2-s2.0-85160502280
来源库	Scopus
引用统计	被引频次[WOS]：0
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/536543
专题	公共分析测试中心
作者单位	1.Bio-X Institutes,Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders,Ministry of Education,Shanghai Jiao Tong University,China 2.Core Research Facilities,Southern University of Science and Technology,Shenzhen,China
推荐引用方式 GB/T 7714	Lin，Muxin,Wang，Ying,Ping，Yong,et al. Simultaneous Multisite Extracellular Electrophysiological Recordings During Electrical Stimulation in Anesthetized Rats[J]. Current protocols,2023,3(5).
APA	Lin，Muxin,Wang，Ying,Ping，Yong,&Chen，Fujun.(2023).Simultaneous Multisite Extracellular Electrophysiological Recordings During Electrical Stimulation in Anesthetized Rats.Current protocols,3(5).
MLA	Lin，Muxin,et al."Simultaneous Multisite Extracellular Electrophysiological Recordings During Electrical Stimulation in Anesthetized Rats".Current protocols 3.5(2023).