The Transcription Factors WRKY41 and WRKY53 Mediate Early Flowering Induced by the Novel Plant Growth Regulator Guvermectin in Arabidopsis thaliana

Yang，Chenyu1; Liu，Chongxi2; Li，Shanshan1; Zhang，Yanyan1; Zhang，Yi3; Wang，Xiangjing2; Xiang，Wensheng1,2

2023-05-01

10.3390/ijms24098424

International Journal of Molecular Sciences   影响因子和分区

1661-6596

1422-0067

Flowering is a crucial stage for plant reproductive success; therefore, the regulation of plant flowering has been widely researched. Although multiple well-defined endogenous and exogenous flowering regulators have been reported, new ones are constantly being discovered. Here, we confirm that a novel plant growth regulator guvermectin (GV) induces early flowering in Arabidopsis. Interestingly, our genetic experiments newly demonstrated that WRKY41 and its homolog WRKY53 were involved in GV-accelerated flowering as positive flowering regulators. Overexpression of WRKY41 or WRKY53 resulted in an early flowering phenotype compared to the wild type (WT). In contrast, the w41/w53 double mutants showed a delay in GV-accelerated flowering. Gene expression analysis showed that flowering regulatory genes SOC1 and LFY were upregulated in GV-treated WT, 35S:WRKY41, and 35S:WRKY53 plants, but both declined in w41/w53 mutants with or without GV treatment. Meanwhile, biochemical assays confirmed that SOC1 and LFY were both direct targets of WRKY41 and WRKY53. Furthermore, the early flowering phenotype of 35S:WRKY41 lines was abolished in the soc1 or lfy background. Together, our results suggest that GV plays a function in promoting flowering, which was co-mediated by WRKY41 and WRKY53 acting as new flowering regulators by directly activating the transcription of SOC1 and LFY in Arabidopsis.

flowering guvermectin LFY SOC1 WRKY41 WRKY53

SCI

英语

其他

National Natural Science Foundation of China[31872037];National Natural Science Foundation of China[32030090];

Biochemistry & Molecular Biology ; Chemistry

Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary

WOS:000987618700001

MDPI

CHEMISTRY

2-s2.0-85159279006

Scopus

1.The State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,100193,China
2.Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee,Northeast Agricultural University,Harbin,No. 600 Changjiang Street, Xiangfang District,150030,China
3.Department of Biology,School of Life Sciences,Institute of Plant and Food Science,Southern University of Science and Technology,Shenzhen,518055,China

Yang，Chenyu,Liu，Chongxi,Li，Shanshan,et al. The Transcription Factors WRKY41 and WRKY53 Mediate Early Flowering Induced by the Novel Plant Growth Regulator Guvermectin in Arabidopsis thaliana[J]. International Journal of Molecular Sciences,2023,24(9).

Yang，Chenyu.,Liu，Chongxi.,Li，Shanshan.,Zhang，Yanyan.,Zhang，Yi.,...&Xiang，Wensheng.(2023).The Transcription Factors WRKY41 and WRKY53 Mediate Early Flowering Induced by the Novel Plant Growth Regulator Guvermectin in Arabidopsis thaliana.International Journal of Molecular Sciences,24(9).

Yang，Chenyu,et al."The Transcription Factors WRKY41 and WRKY53 Mediate Early Flowering Induced by the Novel Plant Growth Regulator Guvermectin in Arabidopsis thaliana".International Journal of Molecular Sciences 24.9(2023).