Rapid exosome isolation and in situ multiplexed detection of exosomal surface proteins and microRNAs on microfluidic platform

Chen，Yulin1; Gao，Dan1; Zhu，Qingyun2; Chu，Bizhu3; Peng，Jie1; Wang，Jian4; Liu，Liping5; Jiang，Yuyang1

2023-04-19

10.1039/d3an00335c

Analyst   影响因子和分区

0003-2654

1364-5528

Exosomes are considered as promising biomarkers for early cancer diagnosis and prognosis. However, the majority of the research studies focused on a single type of exosomal biomarkers, which cannot comprehensively reflect the state of cancer for accurate diagnosis. To address this problem, we presented a ship-shaped microfluidic device containing a microcolumn array for simultaneous in situ detection of exosomal surface proteins and miRNAs. Exosomes were first captured in the microchannels modified with CD63 protein aptamer. Exosomal surface proteins and miRNAs were simultaneously detected in four parallel channels to avoid the interference of fluorescent signals using specific aptamers labeled by Cy5 and catalytic hairpin assembly (CHA) based signal amplification strategy. The limit of detection for multiplexed markers in exosomes was 83 exosomes per μL, which is comparable to previously reported methods. Through quantitative analysis of two disease-specific surface proteins and miRNAs derived from different cancer cells and clinical serum samples, different cancer subtypes as well as cancer patients and healthy people could be significantly distinguished. These results suggest that this simple, highly sensitive, and more accurate analytical strategy by simultaneous in situ profiling of different types of exosomal biomarkers has potential applications in cancer diagnosis and stage monitoring.

SCI

英语

其他

Natural Science Foundation of Guangdong Province["2022A1515011437","2020A1515010660"] ; Shenzhen Fundamental Research and Discipline Layout Project[JCYJ20180508152244835] ; Research and Development Program in Key Areas of Guangdong Province, P.R. China[2019B020209009]

Chemistry

Chemistry, Analytical

WOS:000979096100001

ROYAL SOC CHEMISTRY

CHEMISTRY

2-s2.0-85158899631

Scopus

1.State Key Laboratory of Chemical Oncogenomics,Guangdong Provincial Key Laboratory of Chemical Biology,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen,Guangdong,518055,China
2.The First Affiliated Hospital,Cancer Research Institute,Hengyang Medical School,University of South China,Hengyang,421001,China
3.School of Pharmaceutical Sciences,Health Science Center,Shenzhen University,Shenzhen,518060,China
4.Department of Thoracic Surgery,Shenzhen People's Hospital (The Second Clinical Medical College,Jinan University; The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,Guangdong,518020,China
5.Division of Hepatobiliary and Pancreas Surgery,Department of General Surgery,Shenzhen People's Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern Uni-versity of Science and Technology,Shenzhen,Guangdong,518020,China

Chen，Yulin,Gao，Dan,Zhu，Qingyun,et al. Rapid exosome isolation and in situ multiplexed detection of exosomal surface proteins and microRNAs on microfluidic platform[J]. Analyst,2023,148(10):2387-2394.

Chen，Yulin.,Gao，Dan.,Zhu，Qingyun.,Chu，Bizhu.,Peng，Jie.,...&Jiang，Yuyang.(2023).Rapid exosome isolation and in situ multiplexed detection of exosomal surface proteins and microRNAs on microfluidic platform.Analyst,148(10),2387-2394.

Chen，Yulin,et al."Rapid exosome isolation and in situ multiplexed detection of exosomal surface proteins and microRNAs on microfluidic platform".Analyst 148.10(2023):2387-2394.