Engineering Cell Microenvironment Using Nanopattern-Derived Multicellular Spheroids and Photo-Crosslinked Gelatin/Hyaluronan Hydrogels

Zhang，Zhen1,2; Liu，Yi3; Tao，Xuelian1; Du，Ping1; Enkhbat，Myagmartsend1; Lim，Khoon S.4; Wang，Huaiyu1,2; Wang，Peng Yuan5

2023-04-01

10.3390/polym15081925

Polymers   影响因子和分区

2073-4360

Cell cultures of dispersed cells within hydrogels depict the interaction of the cell–extracellular matrix (ECM) in 3D, while the coculture of different cells within spheroids combines both the effects of cell–cell and cell–ECM interactions. In this study, the cell co-spheroids of human bone mesenchymal stem cells/human umbilical vein endothelial cells (HBMSCs/HUVECs) are prepared with the assistance of a nanopattern, named colloidal self-assembled patterns (cSAPs), which is superior to low-adhesion surfaces. A phenol-modified gelatin/hyaluronan (Gel-Ph/HA-Ph) hydrogel is used to encapsulate the multicellular spheroids and the constructs are photo-crosslinked using blue light. The results show that Gel-Ph/HA-Ph hydrogels with a 5%-to-0.3% ratio have the best properties. Cells in HBMSC/HUVEC co-spheroids are more favorable for osteogenic differentiation (Runx2, ALP, Col1a1 and OPN) and vascular network formation (CD31+ cells) compared to HBMSC spheroids. In a subcutaneous nude mouse model, the HBMSC/HUVEC co-spheroids showed better performance than HBMSC spheroids in angiogenesis and the development of blood vessels. Overall, this study paves a new way for using nanopatterns, cell coculturing and hydrogel technology for the generation and application of multicellular spheroids.

cell spheroids HBMSC HUVEC hydrogel nanopattern

SCI ; EI

英语

其他

Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences[172644KYSB20200002];Bureau of Development and Planning, Chinese Academy of Sciences[172644KYSB20200002];Bureau of Frontier Sciences and Education, Chinese Academy of Sciences[172644KYSB20200002];Beijing Institute of Genomics, Chinese Academy of Sciences[172644KYSB20200002];Bureau of International Cooperation, Chinese Academy of Sciences[172644KYSB20200002];Bureau of Science and Technology for Development, Chinese Academy of Sciences[172644KYSB20200002];CAS Key Laboratory of Digital Earth Science[172644KYSB20200002];CAS Key Laboratory of Human-Machine Intelligence-Synergy Systems, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences[172644KYSB20200002];CAS Key Laboratory of Receptor Research[172644KYSB20200002];Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences[172644KYSB20200002];Center for Ocean Mega-Science, Chinese Academy of Sciences[172644KYSB20200002];Central Asian Center of Drug Discovery and Development, Chinese Academy of Sciences[172644KYSB20200002];Chengdu Institute of Biology[172644KYSB20200002];China-Africa Joint Center for Research and Education, CAS[172644KYSB20200002];Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences[172644KYSB20200002];Chinese Academy of Sciences[172644KYSB20200002];Chinese Academy of Sciences, Shanghai Branch[172644KYSB20200002];Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences[172644KYSB20200002];Fujian Institute of Innovation, Chinese Academy of Sciences[172644KYSB20200002];GuangZhou Institute of Energy Conversion, Chinese Academy of Sciences[172644KYSB20200002];Haixi Institute, Chinese Academy of Sciences[172644KYSB20200002];Hefei Institutes of Physical Science, Chinese Academy of Sciences[172644KYSB20200002];Hefei Science Center, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Solar Activities, Chinese Academy of Sciences[172644KYSB20200002];Institute of Automation, Chinese Academy of Sciences[172644KYSB20200002];Ferring Institute of Reproductive Medicine, Institute of Zoology, Chinese Academy of Sciences[172644KYSB20200002];Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences[172644KYSB20200002];Guangzhou Institute of Geochemistry, Chinese Academy of Sciences[172644KYSB20200002];High Magnetic Field Laboratory, Chinese Academy of Sciences[172644KYSB20200002];Institute of Applied Ecology, Chinese Academy of Sciences[172644KYSB20200002];Institute of Applied Ecology, Chinese Academy of Sciences[172644KYSB20200002];Innovation Academy for Green Manufacture, Chinese Academy of Sciences[172644KYSB20200002];Institute of Atmospheric Physics, Chinese Academy of Sciences[172644KYSB20200002];Institute of Biophysics, Chinese Academy of Sciences[172644KYSB20200002];Institute of Coal Chemistry, Chinese Academy of Sciences[172644KYSB20200002];Institute of Chemistry, Chinese Academy of Sciences[172644KYSB20200002];Institute of Computing Technology, Chinese Academy of Sciences[172644KYSB20200002];Institute of Earth Environment, Chinese Academy of Sciences[172644KYSB20200002];Institute of Geochemistry, Chinese Academy of Sciences[172644KYSB20200002];Institute of Geology and Geophysics, Chinese Academy of Sciences[172644KYSB20200002];Institute of Geodesy and Geophysics, Chinese Academy of Sciences[172644KYSB20200002];Institute of Intelligent Machines, Chinese Academy of Sciences[172644KYSB20200002];Institute of Metal Research, Chinese Academy of Sciences[172644KYSB20200002];Innovation Academy for Seed Design, Chinese Academy of Sciences[172644KYSB20200002];Institute of Acoustics, Chinese Academy of Sciences[172644KYSB20200002];Institute of Oceanology, Chinese Academy of Sciences[172644KYSB20200002];Institute of Semiconductors, Chinese Academy of Sciences[172644KYSB20200002];Institute of Zoology, Chinese Academy of Sciences[172644KYSB20200002];Institute of Plasma Physics, Chinese Academy of Sciences[172644KYSB20200002];Institute of Rock and Soil Mechanics, Chinese Academy of Sciences[172644KYSB20200002];Institute of Software, Chinese Academy of Sciences[172644KYSB20200002];Institute of Soil Science, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Chemistry of Northwestern Plant Resources, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Computational Astrophysics, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Cryogenics, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Engineering Plastics, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Functional Crystals and Laser Technology, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory Of Mental Health, Institute of Psychology, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Renewable Energy and Natural Gas Hydrate, Chinese Academy of Sciences[172644KYSB20200002];Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences[172644KYSB20200002];Institute of Genetics and Developmental Biology, Chinese Academy of Sciences[172644KYSB20200002];Institute of Hydrobiology[172644KYSB20200002];Institute of High Energy Physics[172644KYSB20200002];Institute of Microbiology, Chinese Academy of Sciences[172644KYSB20200002];Institute of Microelectronics of the Chinese Academy of Sciences[172644KYSB20200002];Institute of Botany, Chinese Academy of Sciences[172644KYSB20200002];Institute of Psychology, Chinese Academy of Sciences[172644KYSB20200002];Institut Pasteur of Shanghai, Chinese Academy of Sciences[172644KYSB20200002];Institut Pasteur of Shanghai, Chinese Academy of Sciences[172644KYSB20200002];Institute of Solid State Physics, Chinese Academy of Sciences[172644KYSB20200002];Institute of Urban Environment, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Microelectronic Devices Integrated Technology, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Mollisols Agroecology, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Nanosystem and Hierarchical Fabrication, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Cellulose Chemistry, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Marine Geology and Environment[172644KYSB20200002];Nanjing Institute of Astronomical Optics and Technology, Chinese Academy of Sciences[172644KYSB20200002];National Center for Mathematics and Interdisciplinary Sciences, Chinese Academy of Sciences[172644KYSB20200002];Institute of Remote Sensing and Digital Earth[172644KYSB20200002];Key Laboratory of Cryogenics, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Inorganic Functional Materials and Devices[172644KYSB20200002];Key Laboratory of Particle Astrophysics, Institute of High Energy Physics[172644KYSB20200002];Kunming Institute of Botany, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Synthetic Rubber, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Zoological and Systematics and Evolution, Chinese Academy of Sciences[172644KYSB20200002];Key Laboratory of Zoological Systematics and Evolution, Chinese Academy of Sciences[172644KYSB20200002];Key Research Program of Frontier Science, Chinese Academy of Sciences[172644KYSB20200002];Kunming Institute of Zoology, Chinese Academy of Sciences[172644KYSB20200002];Laboratory of Nano Devices and Applications, Chinese Academy of Sciences[172644KYSB20200002];Shenzhen Key Laboratory of Neuropsychiatric Modulation[172644KYSB20200002];State Key Laboratory of High Temperature Gas Dynamics[172644KYSB20200002];Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences[172644KYSB20200002];State Key Laboratory of Nonlinear Mechanics[172644KYSB20200002];State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences[172644KYSB20200002];National Astronomical Observatories, Chinese Academy of Sciences[172644KYSB20200002];Nanjing Institute of Astronomical Optics and Technology, Chinese Academy of Sciences[172644KYSB20200002];National Center for Mathematics and Interdisciplinary Sciences, Chinese Academy of Sciences[172644KYSB20200002];Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences[172644KYSB20200002];Nanjing Institute of Geology and Paleontology, Chinese Academy of Sciences[172644KYSB20200002];Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences[172644KYSB20200002];National Time Service Center, Chinese Academy of Sciences[172644KYSB20200002];Northwest Institute of Plateau Biology, Chinese Academy of Sciences[172644KYSB20200002];Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences[172644KYSB20200002];Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences[172644KYSB20200002];Anhui Academy of Recycling Economical and Technical Engineering, Chinese Academy of Sciences[172644KYSB20200002];Research Institute of Robotics and Intelligent Manufacturing Innovation, Chinese Academy of Sciences[172644KYSB20200002];Sino-Africa Joint Research Center, Chinese Academy of Sciences[172644KYSB20200002];Shanghai Advanced Research Institute, Chinese Academy of Sciences[172644KYSB20200002];South China Sea Institute of Oceanology, Chinese Academy of Sciences[172644KYSB20200002];Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences[172644KYSB20200002];Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences[172644KYSB20200002];Shanghai Institute of Ceramics, Chinese Academy of Sciences[172644KYSB20200002];Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences[172644KYSB20200002];Shanghai Institute of Materia Medica, Chinese Academy of Sciences[172644KYSB20200002];Suzhou Institute of Nanotechnology, Chinese Academy of Sciences[172644KYSB20200002];Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences[172644KYSB20200002];Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences[172644KYSB20200002];Shanghai Institute of Technical Physics, Chinese Academy of Sciences[172644KYSB20200002];State Key Laboratory of Acoustics[172644KYSB20200002];State Key Laboratory of Drug Research[172644KYSB20200002];State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences[172644KYSB20200002];State Key Laboratory of Microbial Resources[172644KYSB20200002];Wu Wen-Tsun Key Laboratory of Mathematics, Chinese Academy of Sciences[172644KYSB20200002];Wu Wen-Tsun Key Laboratory of Mathematics, Chinese Academy of Sciences[172644KYSB20200002];Wuhan National Biosafety Laboratory[172644KYSB20200002];Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences[172644KYSB20200002];Xi'an Institute of Optics and Precision Mechanics[172644KYSB20200002];Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences[172644KYSB20200002];Youth Innovation Promotion Association of the Chinese Academy of Sciences[172644KYSB20200002];Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences[172644KYSB20200048];Bureau of Frontier Sciences and Education, Chinese Academy of Sciences[172644KYSB20200048];Beijing Institute of Genomics, Chinese Academy of Sciences[172644KYSB20200048];Bureau of Development and Planning, Chinese Academy of Sciences[172644KYSB20200048];Bureau of International Cooperation, Chinese Academy of Sciences[172644KYSB20200048];Bureau of Science and Technology for Development, Chinese Academy of Sciences[172644KYSB20200048];CAS Key Laboratory of Digital Earth Science[172644KYSB20200048];CAS Key Laboratory of Human-Machine Intelligence-Synergy Systems, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences[172644KYSB20200048];CAS Key Laboratory of Receptor Research[172644KYSB20200048];Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences[172644KYSB20200048];Center for Ocean Mega-Science, Chinese Academy of Sciences[172644KYSB20200048];Central Asian Center of Drug Discovery and Development, Chinese Academy of Sciences[172644KYSB20200048];Chengdu Institute of Biology[172644KYSB20200048];China-Africa Joint Center for Research and Education, CAS[172644KYSB20200048];Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences[172644KYSB20200048];Chinese Academy of Sciences[172644KYSB20200048];Chinese Academy of Sciences, Shanghai Branch[172644KYSB20200048];Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences[172644KYSB20200048];Fujian Institute of Innovation, Chinese Academy of Sciences[172644KYSB20200048];GuangZhou Institute of Energy Conversion, Chinese Academy of Sciences[172644KYSB20200048];Haixi Institute, Chinese Academy of Sciences[172644KYSB20200048];Hefei Institutes of Physical Science, Chinese Academy of Sciences[172644KYSB20200048];Hefei Science Center, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Solar Activities, Chinese Academy of Sciences[172644KYSB20200048];Institute of Automation, Chinese Academy of Sciences[172644KYSB20200048];Ferring Institute of Reproductive Medicine, Institute of Zoology, Chinese Academy of Sciences[172644KYSB20200048];Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences[172644KYSB20200048];Guangzhou Institute of Geochemistry, Chinese Academy of Sciences[172644KYSB20200048];High Magnetic Field Laboratory, Chinese Academy of Sciences[172644KYSB20200048];Institute of Applied Ecology, Chinese Academy of Sciences[172644KYSB20200048];Institute of Applied Ecology, Chinese Academy of Sciences[172644KYSB20200048];Innovation Academy for Green Manufacture, Chinese Academy of Sciences[172644KYSB20200048];Institute of Atmospheric Physics, Chinese Academy of Sciences[172644KYSB20200048];Institute of Biophysics, Chinese Academy of Sciences[172644KYSB20200048];Institute of Coal Chemistry, Chinese Academy of Sciences[172644KYSB20200048];Institute of Chemistry, Chinese Academy of Sciences[172644KYSB20200048];Institute of Computing Technology, Chinese Academy of Sciences[172644KYSB20200048];Institute of Earth Environment, Chinese Academy of Sciences[172644KYSB20200048];Institute of Geochemistry, Chinese Academy of Sciences[172644KYSB20200048];Institute of Geology and Geophysics, Chinese Academy of Sciences[172644KYSB20200048];Institute of Geodesy and Geophysics, Chinese Academy of Sciences[172644KYSB20200048];Institute of Intelligent Machines, Chinese Academy of Sciences[172644KYSB20200048];Institute of Metal Research, Chinese Academy of Sciences[172644KYSB20200048];Innovation Academy for Seed Design, Chinese Academy of Sciences[172644KYSB20200048];Institute of Acoustics, Chinese Academy of Sciences[172644KYSB20200048];Institute of Oceanology, Chinese Academy of Sciences[172644KYSB20200048];Institute of Semiconductors, Chinese Academy of Sciences[172644KYSB20200048];Institute of Zoology, Chinese Academy of Sciences[172644KYSB20200048];Institute of Plasma Physics, Chinese Academy of Sciences[172644KYSB20200048];Institute of Rock and Soil Mechanics, Chinese Academy of Sciences[172644KYSB20200048];Institute of Software, Chinese Academy of Sciences[172644KYSB20200048];Institute of Soil Science, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Chemistry of Northwestern Plant Resources, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Computational Astrophysics, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Cryogenics, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Engineering Plastics, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Functional Crystals and Laser Technology, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory Of Mental Health, Institute of Psychology, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Renewable Energy and Natural Gas Hydrate, Chinese Academy of Sciences[172644KYSB20200048];Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences[172644KYSB20200048];Institute of Genetics and Developmental Biology, Chinese Academy of Sciences[172644KYSB20200048];Institute of Hydrobiology[172644KYSB20200048];Institute of High Energy Physics[172644KYSB20200048];Institute of Microbiology, Chinese Academy of Sciences[172644KYSB20200048];Institute of Microelectronics of the Chinese Academy of Sciences[172644KYSB20200048];Institute of Botany, Chinese Academy of Sciences[172644KYSB20200048];Institute of Psychology, Chinese Academy of Sciences[172644KYSB20200048];Institut Pasteur of Shanghai, Chinese Academy of Sciences[172644KYSB20200048];Institut Pasteur of Shanghai, Chinese Academy of Sciences[172644KYSB20200048];Institute of Solid State Physics, Chinese Academy of Sciences[172644KYSB20200048];Institute of Urban Environment, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Microelectronic Devices Integrated Technology, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Mollisols Agroecology, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Nanosystem and Hierarchical Fabrication, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Cellulose Chemistry, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Marine Geology and Environment[172644KYSB20200048];Nanjing Institute of Astronomical Optics and Technology, Chinese Academy of Sciences[172644KYSB20200048];National Center for Mathematics and Interdisciplinary Sciences, Chinese Academy of Sciences[172644KYSB20200048];Institute of Remote Sensing and Digital Earth[172644KYSB20200048];Key Laboratory of Cryogenics, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Inorganic Functional Materials and Devices[172644KYSB20200048];Key Laboratory of Particle Astrophysics, Institute of High Energy Physics[172644KYSB20200048];Kunming Institute of Botany, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Synthetic Rubber, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Zoological and Systematics and Evolution, Chinese Academy of Sciences[172644KYSB20200048];Key Laboratory of Zoological Systematics and Evolution, Chinese Academy of Sciences[172644KYSB20200048];Key Research Program of Frontier Science, Chinese Academy of Sciences[172644KYSB20200048];Kunming Institute of Zoology, Chinese Academy of Sciences[172644KYSB20200048];Laboratory of Nano Devices and Applications, Chinese Academy of Sciences[172644KYSB20200048];Shenzhen Key Laboratory of Neuropsychiatric Modulation[172644KYSB20200048];State Key Laboratory of High Temperature Gas Dynamics[172644KYSB20200048];Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences[172644KYSB20200048];State Key Laboratory of Nonlinear Mechanics[172644KYSB20200048];State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences[172644KYSB20200048];National Astronomical Observatories, Chinese Academy of Sciences[172644KYSB20200048];Nanjing Institute of Astronomical Optics and Technology, Chinese Academy of Sciences[172644KYSB20200048];National Center for Mathematics and Interdisciplinary Sciences, Chinese Academy of Sciences[172644KYSB20200048];Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences[172644KYSB20200048];Nanjing Institute of Geology and Paleontology, Chinese Academy of Sciences[172644KYSB20200048];Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences[172644KYSB20200048];National Time Service Center, Chinese Academy of Sciences[172644KYSB20200048];Northwest Institute of Plateau Biology, Chinese Academy of Sciences[172644KYSB20200048];Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences[172644KYSB20200048];Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences[172644KYSB20200048];Anhui Academy of Recycling Economical and Technical Engineering, Chinese Academy of Sciences[172644KYSB20200048];Research Institute of Robotics and Intelligent Manufacturing Innovation, Chinese Academy of Sciences[172644KYSB20200048];Sino-Africa Joint Research Center, Chinese Academy of Sciences[172644KYSB20200048];Shanghai Advanced Research Institute, Chinese Academy of Sciences[172644KYSB20200048];South China Sea Institute of Oceanology, Chinese Academy of Sciences[172644KYSB20200048];Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences[172644KYSB20200048];Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences[172644KYSB20200048];Shanghai Institute of Ceramics, Chinese Academy of Sciences[172644KYSB20200048];Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences[172644KYSB20200048];Shanghai Institute of Materia Medica, Chinese Academy of Sciences[172644KYSB20200048];Suzhou Institute of Nanotechnology, Chinese Academy of Sciences[172644KYSB20200048];Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences[172644KYSB20200048];Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences[172644KYSB20200048];Shanghai Institute of Technical Physics, Chinese Academy of Sciences[172644KYSB20200048];State Key Laboratory of Acoustics[172644KYSB20200048];State Key Laboratory of Drug Research[172644KYSB20200048];State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences[172644KYSB20200048];State Key Laboratory of Microbial Resources[172644KYSB20200048];Wu Wen-Tsun Key Laboratory of Mathematics, Chinese Academy of Sciences[172644KYSB20200048];Wu Wen-Tsun Key Laboratory of Mathematics, Chinese Academy of Sciences[172644KYSB20200048];Wuhan National Biosafety Laboratory[172644KYSB20200048];Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences[172644KYSB20200048];Xi'an Institute of Optics and Precision Mechanics[172644KYSB20200048];Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences[172644KYSB20200048];Youth Innovation Promotion Association of the Chinese Academy of Sciences[172644KYSB20200048];Guangdong Science and Technology Department[2021A0505030055];Institute for Information Industry, Ministry of Science and Technology, Taiwan[2022YFA1105101];National Natural Science Foundation of China[31870988];National Natural Science Foundation of China[82272157];Science, Technology and Innovation Commission of Shenzhen Municipality[GJHZ20180928115804736];Science and Technology Foundation of Shenzhen City[JSGG20200225152648408];Science, Technology and Innovation Commission of Shenzhen Municipality[ZDSYS20190902093409851];

Polymer Science

Polymer Science

WOS:000979073600001

MDPI

20231814041019

Blood vessels ; Bone ; Cell culture ; Cell engineering ; Endothelial cells ; Stem cells

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Colloid Chemistry:801.3 ; Chemical Products Generally:804

2-s2.0-85154028889

Scopus

1.Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China
2.University of Chinese Academy of Sciences,Beijing,100049,China
3.Shenzhen Key Laboratory of Smart Healthcare Engineering,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
4.School of Medical Sciences,University of Sydney,Sydney,2052,Australia
5.Oujiang Laboratory,Key Laboratory of Alzheimer’s Disease of Zhejiang Province,Institute of Aging,Wenzhou Medical University,Wenzhou,325000,China

Zhang，Zhen,Liu，Yi,Tao，Xuelian,et al. Engineering Cell Microenvironment Using Nanopattern-Derived Multicellular Spheroids and Photo-Crosslinked Gelatin/Hyaluronan Hydrogels[J]. Polymers,2023,15(8).

Zhang，Zhen.,Liu，Yi.,Tao，Xuelian.,Du，Ping.,Enkhbat，Myagmartsend.,...&Wang，Peng Yuan.(2023).Engineering Cell Microenvironment Using Nanopattern-Derived Multicellular Spheroids and Photo-Crosslinked Gelatin/Hyaluronan Hydrogels.Polymers,15(8).

Zhang，Zhen,et al."Engineering Cell Microenvironment Using Nanopattern-Derived Multicellular Spheroids and Photo-Crosslinked Gelatin/Hyaluronan Hydrogels".Polymers 15.8(2023).