Viable but nonculturable (VBNC) state, an underestimated and controversial microbial survival strategy

Liu，Junyan1,2; Yang，Liang3; Kjellerup，Birthe Veno4; Xu，Zhenbo5,6

2023

10.1016/j.tim.2023.04.009

Trends in Microbiology   影响因子和分区

0966-842X

1878-4380

As a unique microbial response to adverse circumstances, the viable but nonculturable (VBNC) state is characterized by the loss of culturability of microbial cells on/in nutrient media that normally support their growth, while maintaining metabolic activity. These cells can resuscitate to a culturable state under suitable conditions. Given the intrinsic importance of the VBNC state and recent debates surrounding it, there is a need to redefine and standardize the term, and to address essential questions such as ‘How to differentiate VBNC from other similar terms?’ and ‘How can VBNC cells be standardly and accurately determined?’. This opinion piece aims at contributing to an improved understanding of the VBNC state and promoting its proper handling as an underestimated and controversial microbial survival strategy.

culturability detection methods persister cells viability viable but nonculturable (VBNC) state

SCI

英语

ESI高被引

其他

National Natural Science Foundation of China[32202199];Higher Education Discipline Innovation Project[B17018];

Biochemistry & Molecular Biology ; Microbiology

Biochemistry & Molecular Biology ; Microbiology

WOS:001076214400001

CELL PRESS

MICROBIOLOGY

2-s2.0-85159891294

Scopus

1.College of Light Industry and Food Science,Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology,Academy of Contemporary Agricultural Engineering Innovations,Zhongkai University of Agriculture and Engineering,Guangzhou,510225,China
2.Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food,Ministry of Agriculture,Guangzhou,510225,China
3.School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China
4.Department of Civil and Environmental Engineering,University of Maryland,College Park,20742,United States
5.School of Food Science and Engineering,Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education,South China University of Technology,Guangzhou,510640,China
6.Department of Laboratory Medicine,the Second Affiliated Hospital of Shantou University Medical College,Shantou,China

Liu，Junyan,Yang，Liang,Kjellerup，Birthe Veno,et al. Viable but nonculturable (VBNC) state, an underestimated and controversial microbial survival strategy[J]. Trends in Microbiology,2023,31(10).

Liu，Junyan,Yang，Liang,Kjellerup，Birthe Veno,&Xu，Zhenbo.(2023).Viable but nonculturable (VBNC) state, an underestimated and controversial microbial survival strategy.Trends in Microbiology,31(10).

Liu，Junyan,et al."Viable but nonculturable (VBNC) state, an underestimated and controversial microbial survival strategy".Trends in Microbiology 31.10(2023).