南方科技大学知识苑(SUSTech KC): CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications

题名	CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications
作者	Huang，Zhen1,2,3 ; Lyon，Christopher J.2,3; Wang，Jin 4; Lu，Shuihua 1; Hu，Tony Y.2,3
通讯作者	Hu，Tony Y.
发表日期	2023
DOI	10.1002/advs.202301697
发表期刊	Advanced Science 影响因子和分区
EISSN	2198-3844
卷号	10 期号:20
摘要	Numerous groups have employed the special properties of CRISPR/Cas systems to develop platforms that have broad potential applications for sensitive and specific detection of nucleic acid (NA) targets. However, few of these approaches have progressed to commercial or clinical applications. This review summarizes the properties of known CRISPR/Cas systems and their applications, challenges associated with the development of such assays, and opportunities to improve their performance or address unmet assay needs using nano-/micro-technology platforms. These include rapid and efficient sample preparation, integrated single-tube, amplification-free, quantifiable, multiplex, and non-NA assays. Finally, this review discusses the current outlook for such assays, including remaining barriers for clinical or point-of-care applications and their commercial development.
关键词	commercialization CRISPR diagnosis nanotechnology point-of-care
相关链接	[Scopus记录]
收录类别	SCI ; EI
语种	英语
学校署名	第一
资助项目	National Institutes of Health["U01CA252965","R01HD090927","R01AI144168","R01HD103511","R21NS130542"] ; Department of Defense[W8IXWH1910926]
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000985047900001
出版者	WILEY
EI入藏号	20232014093241
EI主题词	Nanotechnology ; Nucleic acids
EI分类号	Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Nanotechnology:761 ; Biochemistry:801.2 ; Organic Compounds:804.1
Scopus记录号	2-s2.0-85159094315
来源库	Scopus
引用统计	被引频次[WOS]：13
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/536787
专题	南方科技大学第二附属医院
作者单位	1.National Clinical Research Center for Infectious Diseases,Shenzhen Third People's Hospital,Southern University of Science and Technology,Shenzhen,29 Bulan Road, Guangdong,518112,China 2.Center for Cellular and Molecular Diagnostics,Tulane University School of Medicine,New Orleans,1430 Tulane Ave,70112,United States 3.Department of Biochemistry and Molecular Biology,Tulane University School of Medicine,New Orleans,1430 Tulane Ave,70112,United States 4.Tolo Biotechnology Company Limited,Shanghai,333 Guiping Road,200233,China
第一作者单位	南方科技大学第二附属医院
第一作者的第一单位	南方科技大学第二附属医院
推荐引用方式 GB/T 7714	Huang，Zhen,Lyon，Christopher J.,Wang，Jin,et al. CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications[J]. Advanced Science,2023,10(20).
APA	Huang，Zhen,Lyon，Christopher J.,Wang，Jin,Lu，Shuihua,&Hu，Tony Y..(2023).CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications.Advanced Science,10(20).
MLA	Huang，Zhen,et al."CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications".Advanced Science 10.20(2023).