Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy

Liu，Yongtao1,2; Wen，Shihui2; Wang，Fan3; Zuo，Chao1; Chen，Chaohao4; Zhou，Jiajia2; Jin，Dayong2,5

2023

10.1002/advs.202205990

Advanced Science   影响因子和分区

2198-3844

Upconverting stimulated emission depletion microscopy (U-STED) is emerging as an effective approach for super-resolution imaging due to its significantly low depletion power and its ability to surpass the limitations of the square-root law and achieve higher resolution. Though the compelling performance, a trade-off between the spatial resolution and imaging quality in U-STED has been recognized in restricting the usability due to the low excitation power drove high depletion efficiency. Moreover, it is a burden to search for the right power relying on trial and error as the underpinning mechanism is unknown. Here, a method is proposed that can easily predict the ideal excitation power for high depletion efficiency with the assistance of the non-saturate excitation based on the dynamic cross-relaxation (CR) energy transfer of upconversion nanoparticles. This allows the authors to employ the rate equation model to simulate the populations of each relevant energy state of lanthanides and predict the ideal excitation power for high depletion efficiency. The authors demonstrate that the resolution of STED with the assistance of nonsaturated confocal super-resolution results can easily achieve the highest resolution of sub-40 nm, 1/24 of the excitation wavelengths. The finding on the CR effect provides opportunities for population control in realizing low-power high-resolution nanoscopy.

nonlinear optics imaging stimulated depletion emission microscopy (STED) super-resolution imaging upconversion nanoparticles

SCI ; EI

英语

通讯

National Natural Science Foundation of China (NSFC)[62275125] ; Special project on Cultivating Young Talents of NJUST[30922010313] ; Major Program of the National Natural Science Foundation of China (NSFC)[62227818] ; Australian Research Council Future Fellowships[FT220100018] ; null[FL210100180]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000974391100001

WILEY

20231714004044

Economic and social effects ; Energy transfer ; Excited states ; Nanoparticles ; Optical resolving power ; Stimulated emission

Electromagnetic Waves:711 ; Light/Optics:741.1 ; Nonlinear Optics:741.1.1 ; Nanotechnology:761 ; Atomic and Molecular Physics:931.3 ; Quantum Theory; Quantum Mechanics:931.4 ; Solid State Physics:933 ; Social Sciences:971

2-s2.0-85153325105

Scopus

1.Smart Computational Imaging Laboratory (SCILab),School of Electronic and Optical Engineering,Nanjing University of Science and Technology,Nanjing,Jiangsu Province,210094,China
2.Institute for Biomedical Materials and Devices (IBMD),Faculty of Science,University of Technology Sydney,Sydney,2007,Australia
3.School of Physics,Beihang University,Beijing,102206,China
4.School of Electrical and Data Engineering,Faculty of Engineering and Information Technology,University of Technology Sydney,Sydney,2007,Australia
5.UTS-SUStech Joint Research Centre for Biomedical Materials & Devices,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China

Liu，Yongtao,Wen，Shihui,Wang，Fan,et al. Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy[J]. Advanced Science,2023,10(20).

Liu，Yongtao.,Wen，Shihui.,Wang，Fan.,Zuo，Chao.,Chen，Chaohao.,...&Jin，Dayong.(2023).Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy.Advanced Science,10(20).

Liu，Yongtao,et al."Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy".Advanced Science 10.20(2023).