A Novel Combination of Keratinocyte and Autologous Microskin Grafting to Repair Full-Thickness Skin Loss

Yin，Kai1; Li，Dawei2; Shen，Chuanan2; Shang，Yuru3; Li，Longzhu2; Zhao，Dongxu2; Cheng，Wenfeng2

2022-01-04

10.1159/000526305

European Surgical Research   影响因子和分区

0014-312X

1421-9921

Introduction: The high mortality of patients with extensive deep burns is mainly attributed to the extensive burn wound and the scarce autologous skin left for wound repair. The purpose of this study was to explore how to effectively use the limited remaining autologous skin to repair the extensive deep wound. Methods: Human keratinocytes harvested from the foreskin were cultured and transfected with epidermal growth factors (EGFs) by an adenovirus vector (Ad-EGF). The expression and the biological activity of EGF in both the normal human keratinocytes and the EGF gene-modified human keratinocytes were quantified by ELISA assay and CCK-8 assay, respectively. The differentiated phenotype of epidermal cells was detected by immunofluorescence staining via CK10, CK14, and CK19 expressions. Rats were subjected to a full-thickness skin loss (3.3 cm × 3.0 cm) on the dorsum, which was repaired with the EGF gene-modified human keratinocyte suspension and autologous microskin and covered with the allogeneic skin. The wound healing was quantified, and the expression of EGF mRNA was measured by RT-PCR. Results: The EGF gene-modified human keratinocytes highly expressed EGF. CK10, CK14, and CK19 as keratinocyte differentiation markers were increased in the EGF gene-modified human keratinocytes. Wound healing was accelerated remarkably by the combination of autologous microskin grafting and EGF gene-modified human keratinocytes in vivo, and a very high EGF mRNA expression was observed in EGF gene-modified human keratinocytes groups on days 7 and 14 compared with other groups. Discussion/Conclusion: The EGF gene-modified human keratinocyte suspension may serve as promising seed cells which can effectively secrete EGF to accelerate wound repair in combination with autologous microskin grafting and reduce the autologous skin requirement for wound repair.

Autologous microskin Epidermal growth factor Gene transfection Human keratinocyte suspension Skin defect

SCI

英语

其他

WOS:000839269400001

2-s2.0-85145192607

Scopus

1.Department of Burns and Plastic Surgery,Beijing Jishuitan Hospital,Beijing,China
2.Department of Burns and Plastic Surgery,The Fourth Medical Center of Chinese Pla General Hospital,Beijing,China
3.Department of Plastic Surgery,Southern University of Science and Technology Hospital,Shenzhen,China

Yin，Kai,Li，Dawei,Shen，Chuanan,et al. A Novel Combination of Keratinocyte and Autologous Microskin Grafting to Repair Full-Thickness Skin Loss[J]. European Surgical Research,2022,63(4):269-277.

Yin，Kai.,Li，Dawei.,Shen，Chuanan.,Shang，Yuru.,Li，Longzhu.,...&Cheng，Wenfeng.(2022).A Novel Combination of Keratinocyte and Autologous Microskin Grafting to Repair Full-Thickness Skin Loss.European Surgical Research,63(4),269-277.

Yin，Kai,et al."A Novel Combination of Keratinocyte and Autologous Microskin Grafting to Repair Full-Thickness Skin Loss".European Surgical Research 63.4(2022):269-277.