南方科技大学知识苑(SUSTech KC): Modification in Silver Staining Procedure for Enhanced Protein Staining

题名	Modification in Silver Staining Procedure for Enhanced Protein Staining
作者	Jiang，Yibo; Zheng，Linlin; Lin，Lulin; Lin，Shan ; Xu，Kui; Deng，Sijie; Zhang，Qiqing
发表日期	2022
DOI	10.1155/2022/6243971
发表期刊	BioMed Research International 影响因子和分区
ISSN	2314-6133
EISSN	2314-6141
卷号	2022
摘要	Silver staining is an excellent technique for detecting proteins that are separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Protein silver staining technology has higher sensitivity and is suitable for the detection of low-concentration proteins compared to other staining techniques including the Coomassie brilliant blue detection method. The present study was conducted to enhance the detection ability of the protein staining method. Herein, we modified the recipe of silver staining, a very reproducible method, by adding AMP, PVP, Tween-80, and xylene to enhance the detection ability of protein staining. Furthermore, the particle size and potentiometer were used to detect the particle size and potential difference of the silver ions in the prepared dyeing materials, and then, the morphology, transparency, and size of the dyed silver particles in different dyeing solutions were studied using a transmission electron microscopy (TEM). The obtained results revealed that the use of 0.5% of AMP, PVP, Tween-80, and xylene improved the staining ability of protein silver staining, compared with the original method. Furthermore, 0.5% AMP, 0.5% PVP, 0.5% Tween-80 reagents significantly influenced the morphology, size, potential, and dispersion of silver ions. These results suggested a new idea for further improving the detection ability of protein silver staining.
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	第一
WOS记录号	WOS:000803960600006
Scopus记录号	2-s2.0-85130863901
来源库	Scopus
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/536942
专题	工学院_生物医学工程系南方科技大学第一附属医院
作者单位	Department of Biomedical Engineering,Shenzhen People's Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,Guangdong,518020,China
第一作者单位	生物医学工程系; 南方科技大学第一附属医院
第一作者的第一单位	生物医学工程系; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Jiang，Yibo,Zheng，Linlin,Lin，Lulin,et al. Modification in Silver Staining Procedure for Enhanced Protein Staining[J]. BioMed Research International,2022,2022.
APA	Jiang，Yibo.,Zheng，Linlin.,Lin，Lulin.,Lin，Shan.,Xu，Kui.,...&Zhang，Qiqing.(2022).Modification in Silver Staining Procedure for Enhanced Protein Staining.BioMed Research International,2022.
MLA	Jiang，Yibo,et al."Modification in Silver Staining Procedure for Enhanced Protein Staining".BioMed Research International 2022(2022).