Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis

Zhang, Hailong1; Zhou, Jingwen1; Kou, Xiaoyue1; Liu, Yuqi1; Zhao, Xiaonan1,2; Qin, Guochen3; Wang, Mingyu1; Qian, Guangtao1; Li, Wen1; Huang, Yongshun1; Wang, Xiaoting1; Zhao, Zhenjie1; Li, Shuang4; Wu, Xiaoqian4; Jiang, Lixi2; Feng, Xianzhong5; Zhu, Jian-Kang6,7,8; Li, Lixin1

2023-06-05

10.3389/fpls.2023.1198353

FRONTIERS IN PLANT SCIENCE   影响因子和分区

1664-462X

SYP71, a plant-specific Qc-SNARE with multiple subcellular localization, is essential for symbiotic nitrogen fixation in nodules in Lotus, and is implicated in plant resistance to pathogenesis in rice, wheat and soybean. Arabidopsis SYP71 is proposed to participate in multiple membrane fusion steps during secretion. To date, the molecular mechanism underlying SYP71 regulation on plant development remains elusive. In this study, we clarified that AtSYP71 is essential for plant development and stress response, using techniques of cell biology, molecular biology, biochemistry, genetics, and transcriptomics. AtSYP71-knockout mutant atsyp71-1 was lethal at early development stage due to the failure of root elongation and albinism of the leaves. AtSYP71-knockdown mutants, atsyp71-2 and atsyp71-3, had short roots, delayed early development, and altered stress response. The cell wall structure and components changed significantly in atsyp71-2 due to disrupted cell wall biosynthesis and dynamics. Reactive oxygen species homeostasis and pH homeostasis were also collapsed in atsyp71-2. All these defects were likely resulted from blocked secretion pathway in the mutants. Strikingly, change of pH value significantly affected ROS homeostasis in atsyp71-2, suggesting interconnection between ROS and pH homeostasis. Furthermore, we identified AtSYP71 partners and propose that AtSYP71 forms distinct SNARE complexes to mediate multiple membrane fusion steps in secretory pathway. Our findings suggest that AtSYP71 plays an essential role in plant development and stress response via regulating pH homeostasis through secretory pathway.

AtSYP71 pH homeositasis ROS homeostasis cell wall biosynthesis and dynamics root development vesicle trafficking

SCI

英语

其他

Plant Sciences

Plant Sciences

WOS:001007995700001

FRONTIERS MEDIA SA

Web of Science

1.Northeast Forestry Univ, Coll Life Sci, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Harbin, Peoples R China
2.Zhejiang Univ, Inst Crop Sci, Coll Agr & Biotechnol, Hangzhou, Peoples R China
3.Peking Univ, Inst Adv Agr Sci, Shandong Lab Adv Agr Sci, Weifang, Peoples R China
4.Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China
5.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Changchun, Peoples R China
6.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen, Peoples R China
7.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen, Peoples R China
8.Chinese Acad Agr Sci, Ctr Adv Bioind Technol, Beijing, Peoples R China

Zhang, Hailong,Zhou, Jingwen,Kou, Xiaoyue,et al. Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis[J]. FRONTIERS IN PLANT SCIENCE,2023,14.

Zhang, Hailong.,Zhou, Jingwen.,Kou, Xiaoyue.,Liu, Yuqi.,Zhao, Xiaonan.,...&Li, Lixin.(2023).Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis.FRONTIERS IN PLANT SCIENCE,14.

Zhang, Hailong,et al."Syntaxin of plants71 plays essential roles in plant development and stress response via regulating pH homeostasis".FRONTIERS IN PLANT SCIENCE 14(2023).