MiR-92a expression profiling of exfoliated colonocytes isolated from feces for colorectal cancer screening

Chen, Yixin1,2; He, Wan1,2; Chen, Min3; Zhao, Lizhong4; Shao, Jianyong5,6,7; Lin, Shiyong8; Li, Baoqin9; Xu, Hualin9; Li, Ronghui3; Feng, Jun3; Liang, Ning9; Meng, Yue10,11; Cheng, Xiaojie9; Zhang, Weihua4; Yu, Haoyang9; Xu, Ruilian1,2,12

2023

AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH   影响因子和分区

1943-8141

Objective: This study seeks to assess the efficacy of exfoliated colonocytes isolated from feces (ECIF) miR-92a as a clinical colorectal cancer diagnostic marker in a larger cohort. Methods: Clinicopathologic data from colorectal cancer patients and health controls that underwent colonoscopy, as well as patients of other cancers diagnosed, were included. A total of 963 Chinese participants were enrolled, with 292 (27.4%) having colorectal cancer, 140 (14.5%) having other types of cancer, e.g., pancreatic, liver, oral, bile duct, esophagus, and stomach cancer, 171 (17.8%) having infection in the intestine, rectal, stomach, appendix, and gastrointestinal ulcer, and 360 (37.4%) of healthy controls. ECIF samples were gathered and miR-92a levels were detected using TaqMan probe-based miR-92a real-time quantitative PCR (RT-qPCR) kit developed by Shenzhen GeneBioHealth Co., Ltd. Results: Through a series of experiments, we demonstrated that the Ep-LMB/Vi-LMB magnetic separation system is feasible, highly specific, and highly sensitive at a cutoff value of 1053 copies per 6 ng of ECIF RNA. ECIF miR-92a levels were significantly higher in colorectal cancer patients than in controls. Colorectal cancer detection sensitivity and specificity were 87.3% and 86.9% respectively. Furthermore, the performance of this miR-92a detection kit demonstrated that it is an effective tool for colorectal cancer, with a high sensitivity of 84.1%, even in early cancer stages (0, I, and II). Furthermore, tumor removal resulted in lower stool miR-92a levels (3.21 +/- 0.58 vs. 2.14 +/- 1.14, P < 0.0001, n = 65). Conclusion: Finally, the miR-92a RT-qPCR kit detects ECIF-increased miR-92a and could be used for colorectal cancer screening.

Biomarker colorectal cancer miR-92a screening

SCI

英语

通讯

Guangzhou Science and Technology Program[2018- 03010094] ; Shenzhen Science Program[G20160328103642937] ; National Natural Science Foundation of China[82003149] ; Shenzhen Strategical Emerging Industry De- velopment Grant[JSGG201304121710- 21059] ; Shenzhen Sci- ence and Technology Project[JCYJ201908061- 51610947]

Oncology ; Research & Experimental Medicine

Oncology ; Medicine, Research & Experimental

WOS:000989967800007

E-CENTURY PUBLISHING CORP

Web of Science

1.Jinan Univ, Clin Med Coll 2, Dept Oncol, Shenzhen Peoples Hosp, Guangzhou, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
3.Xiamen Univ, Xiangan Hosp, Xiamen 361102, Fujian, Peoples R China
4.Tianjin Union Med Ctr, Tianjin 300121, Peoples R China
5.Sun Yat Sen Univ, Dept Mol Diagnost, Canc Ctr, Guangzhou 510060, Guangdong, Peoples R China
6.State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China
7.Collaborat Innovat Ctr Canc Med, Guangzhou 510060, Guangdong, Peoples R China
8.Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, Dept Endoscopy Med Oncol Ultrasound & Thorac Surg, State Key Lab Oncol South China,Canc Ctr, Guangzhou 510060, Guangdong, Peoples R China
9.GeneBioHlth Co Ltd, Shenzhen 518055, Guangdong, Peoples R China
10.Guangdong Prov Peoples Hosp, Dept Lab, Guangzhou 510000, Guangdong, Peoples R China
11.Guangdong Acad Med Sci, Guangzhou 510000, Guangdong, Peoples R China
12.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Oncol, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China

Chen, Yixin,He, Wan,Chen, Min,et al. MiR-92a expression profiling of exfoliated colonocytes isolated from feces for colorectal cancer screening[J]. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2023,15(4).

Chen, Yixin.,He, Wan.,Chen, Min.,Zhao, Lizhong.,Shao, Jianyong.,...&Xu, Ruilian.(2023).MiR-92a expression profiling of exfoliated colonocytes isolated from feces for colorectal cancer screening.AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,15(4).

Chen, Yixin,et al."MiR-92a expression profiling of exfoliated colonocytes isolated from feces for colorectal cancer screening".AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH 15.4(2023).