Deep Profiling of the Proteome Dynamics of Pseudomonas aeruginosa Reference Strain PAO1 under Different Growth Conditions

Hou, Mengyun3; Huang, Jingnan1,4; Jia, Tianyuan4; Guan, Yudong3,4; Yang, Fan3; Zhou, Hongchao1; Huang, Piying1; Wang, Jigang1,2,4,5; Yang, Liang4; Dai, Lingyun1,3,4,6

2023-05-22

10.1021/acs.jproteome.2c00785

JOURNAL OF PROTEOME RESEARCH   影响因子和分区

1535-3893

1535-3907

As one of the most common bacterialpathogens causing nosocomialinfections, Pseudomonas aeruginosa ishighly adaptable to survive under various conditions. Here, we profiledthe abundance dynamics of 3489 proteins across different growth stagesin the P. aeruginosa reference strainPAO1 using data-independent acquisition-based quantitative proteomics.The proteins differentially expressed during the planktonic growthexhibit several distinct patterns of expression profiles and are relevantto various biological processes, highlighting the continuous adaptationof the PAO1 proteome during the transition from the acceleration phaseto the stationary phase. By contrasting the protein expressions ina biofilm to planktonic cells, the known roles of T6SS, phenazinebiosynthesis, quorum sensing, and c-di-GMP signaling in the biofilmformation process were confirmed. Additionally, we also discoveredseveral new functional proteins that may play roles in the biofilmformation process. Lastly, we demonstrated the general concordanceof protein expressions within operons across various growth states,which permits the study of coexpression protein units, and reversely,the study of regulatory components in the operon structure. Takentogether, we present a high-quality and valuable resource on the proteomicdynamics of the P. aeruginosa referencestrain PAO1, with the potential of advancing our understanding ofthe overall physiology of Pseudomonas bacteria.

Pseudomonas aeruginosa PAO1 microbial proteomics planktonic culture biofilm data-independentacquisition protein abundance differential expression operon

SCI

英语

通讯

National Natural Science Foundation of China["82003814","32070748"] ; Excellent Scientific and Technological Innovation Training Program of Shenzhen[RCYX20210706092040048] ; Guangdong Natural Science Foundation for Distinguished Young Scholar[2020B1515020003]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:001004507000001

AMER CHEMICAL SOC

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Dept Nephrol, Shenzhen Key Lab Kidney Dis,Clin Med Coll 2, Shenzhen 518020, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
3.Jinan Univ, Shenzhen Inst Resp Dis, Shenzhen Peoples Hosp, Clin Med Coll 2, Shenzhen 518020, Peoples R China
4.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Guangdong, Peoples R China
5.Chinese Acad Chinese Med Sci, Inst Chinese Mat Med, Artemisinin Res Ctr, Beijing 100700, Peoples R China
6.ASTAR, Inst Mol & Cell Biol, Singapore 138673, Singapore

Hou, Mengyun,Huang, Jingnan,Jia, Tianyuan,et al. Deep Profiling of the Proteome Dynamics of Pseudomonas aeruginosa Reference Strain PAO1 under Different Growth Conditions[J]. JOURNAL OF PROTEOME RESEARCH,2023,22(6).

Hou, Mengyun.,Huang, Jingnan.,Jia, Tianyuan.,Guan, Yudong.,Yang, Fan.,...&Dai, Lingyun.(2023).Deep Profiling of the Proteome Dynamics of Pseudomonas aeruginosa Reference Strain PAO1 under Different Growth Conditions.JOURNAL OF PROTEOME RESEARCH,22(6).

Hou, Mengyun,et al."Deep Profiling of the Proteome Dynamics of Pseudomonas aeruginosa Reference Strain PAO1 under Different Growth Conditions".JOURNAL OF PROTEOME RESEARCH 22.6(2023).