Identification of a novel HIV-1 second-generation circulating recombinant form (CRF136_0107) among MSM in China

Zhang, Yun-Qi1,2; Li, Qing-Hai3; Li, En-Long1,2; Wang, Yi-Ru1,2; Tang, Zi-Yan1,2; Gao, Xin1,2; Lu, Ran-Ran3; Liu, Si-Yu4; Chen, Xiao-Hong4; Wang, Fu-Xiang4,5; Wang, Jia-Ye1,2,6

2023-07-01

10.1097/QAD.0000000000003589

AIDS   影响因子和分区

0269-9370

1473-5571

Objective:To investigate the molecular epidemiology of HIV-1 in Heilongjiang, China, and try to spot signs of new circulating recombinant form (CRF) in this region.Design:A molecular epidemiological study was conducted in Heilongjiang, China during 2011-2020.Methods:Plasma samples were collected from three HIV-1-positive patients (two MSM and one man lacking risk factor information). The near full-length genome sequences (NFLGs) of a novel CRF were then obtained and subjected to phylogenetic analysis using Mega 7.0.26. Recombination analysis was performed by the jumping profile Hidden Markov Model (jpHMM). Finally, the origin time of this novel CRF was inferred using the Bayesian phylogenetic analysis in Beast v1.10.4.Results:The three NFLGs formed a distinct monophyletic cluster in the neighbor-joining (NJ) tree. Recombination analysis revealed that the recombinant genome was composed of five segments derived from CRF01_AE, subtypes B, and C, but further confirmed to be a second-generation recombinant form of CRF01_AE/CRF07_BC by a comparison of genome maps and subregion phylogenetic analysis and, therefore, designated as CRF136_0107. With Bayesian phylogenetic analysis, CRF136_0107 was estimated to originate around 2010-2011.Conclusion:A novel HIV-1 CRF01_AE/CRF07_BC second-generation CRF called CRF136_0107 was identified among MSM in Heilongjiang, a northeast province of China.

CRF01_AE CRF07_BC CRF136_0107 HIV-1 MSM

SCI

英语

其他

National Natural Science Foundations of China["81601755","81971915"] ; Natural Science Foundation of Heilongjiang Province of China[LH2022H048] ; Innovation and Entrepreneurship Training Program for College Students of Harbin Medical University[202110226119]

Immunology ; Infectious Diseases ; Virology

Immunology ; Infectious Diseases ; Virology

WOS:001000274000002

LIPPINCOTT WILLIAMS & WILKINS

IMMUNOLOGY

Web of Science

1.Harbin Med Univ, Dept Microbiol, Harbin, Peoples R China
2.Harbin Med Univ, Heilongjiang Prov Key Lab Infect & Immun, Harbin, Peoples R China
3.Harbin Med Univ, Coll Pharm, Genom Res Ctr, Harbin, Peoples R China
4.Harbin Med Univ, Affiliated Hosp 4, Dept Infect Dis, Harbin, Peoples R China
5.Southern Univ Sci & Technol, Affiliated Hosp 2, Dept Infect Dis, Shenzhen, Peoples R China
6.Harbin Med Univ, Dept Microbiol, 157 Baojian Rd, Harbin 150001, Peoples R China

Zhang, Yun-Qi,Li, Qing-Hai,Li, En-Long,et al. Identification of a novel HIV-1 second-generation circulating recombinant form (CRF136_0107) among MSM in China[J]. AIDS,2023,37(8).

Zhang, Yun-Qi.,Li, Qing-Hai.,Li, En-Long.,Wang, Yi-Ru.,Tang, Zi-Yan.,...&Wang, Jia-Ye.(2023).Identification of a novel HIV-1 second-generation circulating recombinant form (CRF136_0107) among MSM in China.AIDS,37(8).

Zhang, Yun-Qi,et al."Identification of a novel HIV-1 second-generation circulating recombinant form (CRF136_0107) among MSM in China".AIDS 37.8(2023).