南方科技大学知识苑(SUSTech KC): microRNA-130b May Induce Cerebral Vasospasm after Subarachnoid Hemorrhage via Modulating Kruppel-like Factor 4

题名	microRNA-130b May Induce Cerebral Vasospasm after Subarachnoid Hemorrhage via Modulating Kruppel-like Factor 4
作者	Huang, Zewei 1; Hu, Jiliang 2,3; Xu, Jiongfu 2; Wang, Hao2,3 ; Dai, Limeng 2
通讯作者	Wang, Hao; Dai, Limeng
发表日期	2023-06-01
DOI	10.1080/10985549.2023.2210030
发表期刊	MOLECULAR AND CELLULAR BIOLOGY 影响因子和分区
ISSN	0270-7306
EISSN	1098-5549
卷号	43 期号:7
摘要	Recently, the diverse functions of microRNAs (miRNAs) in brain diseases have been demonstrated. We intended to uncover the functional role of microRNA-130b (miR-130b) in cerebral vasospasm (CVS) following subarachnoid hemorrhage (SAH). SAH was induced by injecting the autologous blood into the cisterna magna of Sprague Dawley rats. The cerebral vascular smooth muscle cells (cVSMCs) were extracted for in vitro experimentation. In vitro and in vivo assays were implemented with transfection of miR-130b mimic/inhibitor, sh-Kruppel-like factor 4 (KLF4), oe-KLF4 plasmids or p38/MAPK signaling pathway agonist (anisomycin), respectively, to elaborate the role of miR-130b in CVS following SAH. Elevated miR-130b and reduced KLF4 were found in SAH patients and rat models of SAH. KLF4 was the target gene of miR-130b. miR-130b promoted the proliferation and migration of cVSMCs through the Inhibition of KLF4. Besides, KLF4 inhibited the proliferation and migration of cVSMCs through blockage of the p38/MAPK pathway. Furthermore, in vivo assay confirmed the inhibitory effect of decreased miR-130b in CVS following SAH. In conclusion, miR-130b may activate the p38/MAPK signaling pathway through targeted inhibition of KLF4, thereby contributing to some extent to the development of cerebral vasospasm after SAH.
关键词	microRNA-130b cerebral vasospasm subarachnoid hemorrhage KLF4 p38 MAPK signaling pathway
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Shenzhen Key Laboratory of Prevention and Treatment of Severe Infections[ZDSYS20200811142804014] ; Shenzhen Key Medical Discipline Construction Fund[SZXK045] ; General project of Shenzhen Nature Fund["JCYJ20210324114008023","2022N070"]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:001017611500001
出版者	TAYLOR & FRANCIS INC
ESI学科分类	MOLECULAR BIOLOGY & GENETICS
来源库	Web of Science
引用统计	被引频次[WOS]：2
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/549191
专题	南方科技大学第一附属医院
作者单位	1.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp,Clin Med Coll 2, Affiliated Hosp 1,Dept Crit Care Med, Shenzhen, Peoples R China 2.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Dept Neurosurg,Clin Med Coll 2,Affiliated Hosp 1, Shenzhen, Peoples R China 3.Guangdong Engn Technol Res Ctr Nervous Anat & Rela, Shenzhen, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Huang, Zewei,Hu, Jiliang,Xu, Jiongfu,et al. microRNA-130b May Induce Cerebral Vasospasm after Subarachnoid Hemorrhage via Modulating Kruppel-like Factor 4[J]. MOLECULAR AND CELLULAR BIOLOGY,2023,43(7).
APA	Huang, Zewei,Hu, Jiliang,Xu, Jiongfu,Wang, Hao,&Dai, Limeng.(2023).microRNA-130b May Induce Cerebral Vasospasm after Subarachnoid Hemorrhage via Modulating Kruppel-like Factor 4.MOLECULAR AND CELLULAR BIOLOGY,43(7).
MLA	Huang, Zewei,et al."microRNA-130b May Induce Cerebral Vasospasm after Subarachnoid Hemorrhage via Modulating Kruppel-like Factor 4".MOLECULAR AND CELLULAR BIOLOGY 43.7(2023).