Distribution of drug-resistant genes in alveolar lavage fluid from patients with psittacosis and traceability analysis of causative organisms

Lu, Huiqun1; Yuan, Jing2; Wu, Zeming3; Wang, Lingwei4; Wu, Shuang5; Chen, Qiongcheng5; Zhang, Zhen5; Chen, Zhigao5; Zou, Xuan5; Hu, Qinghua5; Feng, Tiejian5; Lu, Jianhua5; Ji, Liyin5; Qiu, Shuxiang1; Xu, Shiqin5; Jiang, Min5; Li, Yinghui5; Peng, Bo5; Bai, Qinqin1; Cai, Rui5; Geng, Yijie5; Shi, Xiaolu1,5

2023-06-22

10.3389/fmicb.2023.1182604

FRONTIERS IN MICROBIOLOGY   影响因子和分区

1664-302X

BackgroundChlamydia psittaci is a small bacterium often found in birds, including poultry, and domesticated mammals, which causes psittacosis (or parrot fever) in humans. Different strains of C. psittaci respond variably to antibiotics, suggesting a possible risk of antibiotic resistance. In general, different genotypes of C. psittaci have relatively stable hosts and different pathogenicity. MethodsMacrogenomic sequencing was performed using nucleic acids extracted from psittacosis patients' alveolar lavage fluid samples and analyzed for genetic variability and antibiotic resistance genes. Nucleic acid amplification sequences specific to the core coding region of the C. psittaci ompA gene were used, and a phylogenetic tree was constructed with C. psittaci genotypic sequences from other sources, including Chinese published sources. The C. psittaci found in each patient were genotyped by comparing ompA gene sequences. In addition, to better illustrate the relationship between genotype and host of C. psittaci, 60 bird fecal samples were collected from bird-selling stores for screening and C. psittaci typing. ResultsMacrogenomic sequence alignment revealed the presence of resistance genes in varying abundance in samples from all three patients, including C. psittaci resistance gene sequences from two patients that matched those previously published on NCBI. Based on ompA genotyping, two patients were infected with C. psittaci genotype A and one patient was infected with genotype B. All five C. psittaci-positive samples obtained from bird-selling stores were genotype A. Both genotypes are reported to be infectious to humans. The host origin of the samples and the previously reported main sources of each genotype suggested that all but one of the C. psittaci genotype A in this study were derived from parrots, while genotype B was probably derived from chickens. ConclusionThe presence of bacterial resistance genes in psittacosis patients may affect the efficacy of clinical antibiotic therapy. Focusing on the developmental progression of bacterial resistance genes and differences in the therapeutic efficacy may facilitate effective treatment of clinical bacterial infections. Pathogenicity genotypes (e.g., genotype A and genotype B) are not limited to one animal host, suggesting that monitoring the development and changes of C. psittaci may help prevent transmission to humans.

psittacosis metagenomic next-generation sequencing genotyping host traceability antibiotics

SCI

英语

其他

National Natural Science Foundation of China[81773436] ; Key Scientific and Technological Project of Shenzhen Science and Technology Innovation Committee[KCXFZ202002011006190] ; Shenzhen Key Medical Discipline Construction Fund[SZXK064] ; Special anti-epidemic project of Shenzhen Science and Technology Innovation Committee[JSGG20220301090005007] ; Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences[2020-PT330-006] ; Sanming Project of Medicine in Shenzhen[SZSM201811071] ; Research Foundation of Shenzhen Science and Technology Emergency Key Technology Program[JSGG20220301090007009] ; Science and Technology Planning Project of Guangdong Province of China[2021 B1212030009]

Microbiology

Microbiology

WOS:001023106600001

FRONTIERS MEDIA SA

Web of Science

1.Univ South China, Sch Publ Hlth, Dept Publ Hlth Lab Sci, Hengyang, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Affiliated Hosp 2, Shenzhen, Peoples R China
3.Guangzhou Univ Chinese Med Futian, Shenzhen Hosp, Shenzhen, Peoples R China
4.Shenzhen Peoples Hosp, Shenzhen, Peoples R China
5.Shenzhen Ctr Dis Control & Prevent, Shenzhen, Peoples R China

Lu, Huiqun,Yuan, Jing,Wu, Zeming,et al. Distribution of drug-resistant genes in alveolar lavage fluid from patients with psittacosis and traceability analysis of causative organisms[J]. FRONTIERS IN MICROBIOLOGY,2023,14.

Lu, Huiqun.,Yuan, Jing.,Wu, Zeming.,Wang, Lingwei.,Wu, Shuang.,...&Shi, Xiaolu.(2023).Distribution of drug-resistant genes in alveolar lavage fluid from patients with psittacosis and traceability analysis of causative organisms.FRONTIERS IN MICROBIOLOGY,14.

Lu, Huiqun,et al."Distribution of drug-resistant genes in alveolar lavage fluid from patients with psittacosis and traceability analysis of causative organisms".FRONTIERS IN MICROBIOLOGY 14(2023).