题名 | Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens |
作者 | |
通讯作者 | Pijlman, Gorben P. |
发表日期 | 2023-05-24
|
DOI | |
发表期刊 | |
EISSN | 1664-302X
|
卷号 | 14 |
摘要 | Usutu virus (USUV) is a mosquito-borne flavivirus of African origin. Over the past decades, USUV has spread through Europe causing mass die-offs among multiple bird species. The natural transmission cycle of USUV involves Culex spp. mosquitoes as vectors and birds as amplifying hosts. Next to birds and mosquitoes, USUV has also been isolated from multiple mammalian species, including humans, which are considered dead-end hosts. USUV isolates are phylogenetically classified into an African and European branch, subdivided into eight genetic lineages (Africa 1, 2, and 3 and Europe 1, 2, 3, 4, and 5 lineages). Currently, multiple African and European lineages are co-circulating in Europe. Despite increased knowledge of the epidemiology and pathogenicity of the different lineages, the effects of co-infection and transmission efficacy of the co-circulating USUV strains remain unclear. In this study, we report a comparative study between two USUV isolates as follows: a Dutch isolate (USUV-NL, Africa lineage 3) and an Italian isolate (USUV-IT, Europe lineage 2). Upon co-infection, USUV-NL was consistently outcompeted by USUV-IT in mosquito, mammalian, and avian cell lines. In mosquito cells, the fitness advantage of USUV-IT was most prominently observed in comparison to the mammalian or avian cell lines. When Culex pipiens mosquitoes were orally infected with the different isolates, no overall differences in vector competence for USUV-IT and USUV-NL were observed. However, during the in vivo co-infection assay, it was observed that USUV-NL infectivity and transmission were negatively affected by USUV-IT but not vice versa. |
关键词 | |
相关链接 | [来源记录] |
收录类别 | |
语种 | 英语
|
学校署名 | 其他
|
资助项目 | program One-Health PACT - Dutch Research Council (NWO)[109986]
; ZonMW[522003001]
; European Union[734548]
|
WOS研究方向 | Microbiology
|
WOS类目 | Microbiology
|
WOS记录号 | WOS:001002646400001
|
出版者 | |
来源库 | Web of Science
|
引用统计 |
被引频次[WOS]:0
|
成果类型 | 期刊论文 |
条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/549665 |
专题 | 南方科技大学医学院_公共卫生及应急管理学院 |
作者单位 | 1.Wageningen Univ & Res, Lab Virol, Wageningen, Netherlands 2.Wageningen Univ & Res, Lab Entomol, Wageningen, Netherlands 3.Southern Univ Sci & Technol SUSTech, Sch Publ Hlth & Emergency Management, Shenzhen, Peoples R China |
推荐引用方式 GB/T 7714 |
van Bree, Joyce W. M.,Linthout, Charlotte,van Dijk, Teije,et al. Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens[J]. FRONTIERS IN MICROBIOLOGY,2023,14.
|
APA |
van Bree, Joyce W. M..,Linthout, Charlotte.,van Dijk, Teije.,Abbo, Sandra R..,Fros, Jelke J..,...&Wang, Haidong.(2023).Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens.FRONTIERS IN MICROBIOLOGY,14.
|
MLA |
van Bree, Joyce W. M.,et al."Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens".FRONTIERS IN MICROBIOLOGY 14(2023).
|
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | 操作 | |
compe.pdf(1455KB) | -- | -- | 开放获取 | -- | 浏览 |
|
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论