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题名

Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens

作者
通讯作者Pijlman, Gorben P.
发表日期
2023-05-24
DOI
发表期刊
EISSN
1664-302X
卷号14
摘要

Usutu virus (USUV) is a mosquito-borne flavivirus of African origin. Over the past decades, USUV has spread through Europe causing mass die-offs among multiple bird species. The natural transmission cycle of USUV involves Culex spp. mosquitoes as vectors and birds as amplifying hosts. Next to birds and mosquitoes, USUV has also been isolated from multiple mammalian species, including humans, which are considered dead-end hosts. USUV isolates are phylogenetically classified into an African and European branch, subdivided into eight genetic lineages (Africa 1, 2, and 3 and Europe 1, 2, 3, 4, and 5 lineages). Currently, multiple African and European lineages are co-circulating in Europe. Despite increased knowledge of the epidemiology and pathogenicity of the different lineages, the effects of co-infection and transmission efficacy of the co-circulating USUV strains remain unclear. In this study, we report a comparative study between two USUV isolates as follows: a Dutch isolate (USUV-NL, Africa lineage 3) and an Italian isolate (USUV-IT, Europe lineage 2). Upon co-infection, USUV-NL was consistently outcompeted by USUV-IT in mosquito, mammalian, and avian cell lines. In mosquito cells, the fitness advantage of USUV-IT was most prominently observed in comparison to the mammalian or avian cell lines. When Culex pipiens mosquitoes were orally infected with the different isolates, no overall differences in vector competence for USUV-IT and USUV-NL were observed. However, during the in vivo co-infection assay, it was observed that USUV-NL infectivity and transmission were negatively affected by USUV-IT but not vice versa.

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英语
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其他
资助项目
program One-Health PACT - Dutch Research Council (NWO)[109986] ; ZonMW[522003001] ; European Union[734548]
WOS研究方向
Microbiology
WOS类目
Microbiology
WOS记录号
WOS:001002646400001
出版者
来源库
Web of Science
引用统计
被引频次[WOS]:0
成果类型期刊论文
条目标识符http://sustech.caswiz.com/handle/2SGJ60CL/549665
专题南方科技大学医学院_公共卫生及应急管理学院
作者单位
1.Wageningen Univ & Res, Lab Virol, Wageningen, Netherlands
2.Wageningen Univ & Res, Lab Entomol, Wageningen, Netherlands
3.Southern Univ Sci & Technol SUSTech, Sch Publ Hlth & Emergency Management, Shenzhen, Peoples R China
推荐引用方式
GB/T 7714
van Bree, Joyce W. M.,Linthout, Charlotte,van Dijk, Teije,et al. Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens[J]. FRONTIERS IN MICROBIOLOGY,2023,14.
APA
van Bree, Joyce W. M..,Linthout, Charlotte.,van Dijk, Teije.,Abbo, Sandra R..,Fros, Jelke J..,...&Wang, Haidong.(2023).Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens.FRONTIERS IN MICROBIOLOGY,14.
MLA
van Bree, Joyce W. M.,et al."Competition between two Usutu virus isolates in cell culture and in the common house mosquito Culex pipiens".FRONTIERS IN MICROBIOLOGY 14(2023).
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