Fiber Laser-Based Lasso-Shaped Biosensor for High Precision Detection of Cancer Biomarker-CEACAM5 in Serum

Hu, Jie1; Song, Enlai2; Liu, Yuhui1; Yang, Qiaochu2; Sun, Junhui1; Chen, Jinna1; Meng, Yue3; Jia, Yanwei4; Yu, Zhiguang5; Ran, Yang2; Shao, Liyang1; Shum, Perry Ping1

2023-07-01

10.3390/bios13070674

BIOSENSORS-BASEL   影响因子和分区

2079-6374

Detection of trace tumor markers in blood/serum is essential for the early screening and prognosis of cancer diseases, which requires high sensitivity and specificity of the assays and biosensors. A variety of label-free optical fiber-based biosensors has been developed and yielded great opportunities for Point-of-Care Testing (POCT) of cancer biomarkers. The fiber biosensor, however, suffers from a compromise between the responsivity and stability of the sensing signal, which would deteriorate the sensing performance. In addition, the sophistication of sensor preparation hinders the reproduction and scale-up fabrication. To address these issues, in this study, a straightforward lasso-shaped fiber laser biosensor was proposed for the specific determination of carcinoembryonic antigen (CEA)-related cell adhesion molecules 5 (CEACAM5) protein in serum. Due to the ultra-narrow linewidth of the laser, a very small variation of lasing signal caused by biomolecular bonding can be clearly distinguished via high-resolution spectral analysis. The limit of detection (LOD) of the proposed biosensor could reach 9.6 ng/mL according to the buffer test. The sensing capability was further validated by a human serum-based cancer diagnosis trial, enabling great potential for clinical use. The high reproduction of fabrication allowed the mass production of the sensor and extended its utility to a broader biosensing field.

optical fiber biosensor fiber laser lasso structure CEACAM5 protein human serum

SCI ; EI

英语

第一 ; 通讯

Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation

Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation

WOS:001035070200001

MDPI

20233114483197

Biomarkers ; Biosensors ; Cell adhesion ; Cell proliferation ; Diagnosis ; Diseases ; Fiber lasers ; Optical fiber fabrication ; Optical fibers ; Spectrum analysis ; Tumors

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Fiber Optics:741.1.2 ; Solid State Lasers:744.4 ; Organic Compounds:804.1

Web of Science

1.Southern Univ Sci & Technol, Dept Elect & Elect Engn, Shenzhen 518055, Peoples R China
2.Jinan Univ, Inst Photon Technol, Guangdong Prov Key Lab Opt Fiber Sensing & Commun, Guangzhou 510632, Peoples R China
3.Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Dept Clin Lab, Guangzhou 511436, Peoples R China
4.Univ Macau, Inst Microelect, Fac Sci & Technol ECE, Fac Hlth Sci,State Key Lab Analog & Mixed Signal V, Macau 999078, Peoples R China
5.Medcaptain Med Technol Co Ltd, Shenzhen 518055, Peoples R China

Hu, Jie,Song, Enlai,Liu, Yuhui,et al. Fiber Laser-Based Lasso-Shaped Biosensor for High Precision Detection of Cancer Biomarker-CEACAM5 in Serum[J]. BIOSENSORS-BASEL,2023,13(7).

Hu, Jie.,Song, Enlai.,Liu, Yuhui.,Yang, Qiaochu.,Sun, Junhui.,...&Shum, Perry Ping.(2023).Fiber Laser-Based Lasso-Shaped Biosensor for High Precision Detection of Cancer Biomarker-CEACAM5 in Serum.BIOSENSORS-BASEL,13(7).

Hu, Jie,et al."Fiber Laser-Based Lasso-Shaped Biosensor for High Precision Detection of Cancer Biomarker-CEACAM5 in Serum".BIOSENSORS-BASEL 13.7(2023).