Eliminated colistin-resistance dissemination by a carbon nanotube-mediated CRISPR/Cas9 system - a combined effect of curing plasmid and conjugation blocking

Yu, Kaiqiang1,2,3; Cheng, Zhanwen1; Sun, Yuhong1; Ren, Hefeng1; Chen, Hong1,2,3; Xia, Yu1,2,3

2023-07-01

10.1039/d3en00145h

ENVIRONMENTAL SCIENCE-NANO   影响因子和分区

2051-8153

2051-8161

The spread of antibiotic resistance genes (ARGs) has become a major public health problem. The recent global spread of colistin-resistance gene mcr-1 via conjugative plasmids threatens the utility of colistin as the 'last-resort' antibiotic against Gram-negative pathogens. Despite the fact that multiple CRISPR/Cas9 systems targeting mcr-1 have been proposed, their poor delivery efficiency constrains effective eradication of the colistin-resistance trait. Herein, we demonstrated an efficient CRISPR/Cas delivery into wild colistin-resistant Escherichia coli (E. coli) using modified single-wall carbon nanotubes (SWCNTs). Our results revealed that the SWCNT-CRISPR complex could not only cure colistin-resistance but also inhibit the conjugation of mcr-1 plasmids. The dual function is mainly attributed to i) targeted mcr-1 cleaving activity by importing CRISPR/Cas9 systems; ii) the shortage of ATP supply for conjugation due to a disordered electron transfer chain by conductive SWCNTs. Our work demonstrates the feasibility of utilizing SWCNTs to facilitate CRISPR-based resistance eradication, opening up new possibilities for combating environmental antibiotic resistance with nanotechnology.

SCI ; EI

英语

第一 ; 通讯

National Key Research and Development Program of China[2021YFA1202500] ; National Natural Science Foundation of China["42107257","42007216"]

Chemistry ; Environmental Sciences & Ecology ; Science & Technology - Other Topics

Chemistry, Multidisciplinary ; Environmental Sciences ; Nanoscience & Nanotechnology

WOS:001035931700001

ROYAL SOC CHEMISTRY

20233214519713

Curing ; Escherichia coli ; Genes ; Single-walled carbon nanotubes (SWCN)

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Nanotechnology:761 ; Chemical Reactions:802.2 ; Crystalline Solids:933.1

Web of Science

1.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut C, Shenzhen 518055, Peoples R China

Yu, Kaiqiang,Cheng, Zhanwen,Sun, Yuhong,et al. Eliminated colistin-resistance dissemination by a carbon nanotube-mediated CRISPR/Cas9 system - a combined effect of curing plasmid and conjugation blocking[J]. ENVIRONMENTAL SCIENCE-NANO,2023,10(9):2387-2398.

Yu, Kaiqiang,Cheng, Zhanwen,Sun, Yuhong,Ren, Hefeng,Chen, Hong,&Xia, Yu.(2023).Eliminated colistin-resistance dissemination by a carbon nanotube-mediated CRISPR/Cas9 system - a combined effect of curing plasmid and conjugation blocking.ENVIRONMENTAL SCIENCE-NANO,10(9),2387-2398.

Yu, Kaiqiang,et al."Eliminated colistin-resistance dissemination by a carbon nanotube-mediated CRISPR/Cas9 system - a combined effect of curing plasmid and conjugation blocking".ENVIRONMENTAL SCIENCE-NANO 10.9(2023):2387-2398.