PLA-HPG based coating enhanced anti-biofilm and wound healing of Shikonin in MRSA-infected burn wound

Han, Huiyu1; Chen, Lianheng1; Liang, Shu2,3; Lu, Jiawei1; Wu, Yashi1; Wang, Xiongjun1; Xu, Fei4; Ge, Lanlan2; Xiao, Lingyun1,2

2023-08-10

10.3389/fbioe.2023.1243525

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY   影响因子和分区

2296-4185

Burn wounds are susceptible to bacterial infections, including Methicillin-resistant Staphylococcus aureus (MRSA), which typically form biofilms and exhibit drug resistance. They also have specific feature of abundant exudate, necessitating frequent drug administration. Shikonin (SKN) has been reported to reverse MRSA drug resistance and possesses anti-biofilm and wound healing properties, however, it suffers from drawbacks of low solubility and instability. In this study, we developed PLA-HPG based bioadhesive nanoparticles SKN/BNP, which demonstrated a drug loading capacity of about 3.6%, and exhibited sustained-release behavior of SKN. The aldehyde groups present on the surface of BNP improved the local adhesion of SKN/BNP both in vitro and in vivo, thereby reducing the frequency of drug dosing in exudate-rich burn wounds. BNP alone enhanced proliferation and migration of the fibroblast, while SKN/BNP promoted fibroblast proliferation and migration as well as angiogenesis. Due to its bioadhesive property, BNP directly interacted with biofilm and enhanced the efficacy of SKN against MRSA biofilm in vitro. In a mouse model of MRSA-infected burn wounds, SKN/BNP demonstrated improved anti-biofilm and wound healing efficiency. Overall, our findings suggest that SKN/BNP holds great promise as a novel and effective treatment option for clinical applications in MRSA-infected burn wounds.

PLA-HPG bioadhesive nanoparticles Shikonin MRSA biofilm burn wound

SCI

英语

通讯

This work is funded by the Shenzhen Science and Technology Program (Grants #: JCYJ20190806153401647 and 20190806151816859), and the National Natural Science Foundation of China (Grants #: 81903914 and 81903760).["20190806151816859","81903914"] ; Shenzhen Science and Technology Program[81903760] ; null[JCYJ20190806153401647]

Biotechnology & Applied Microbiology ; Science & Technology - Other Topics

Biotechnology & Applied Microbiology ; Multidisciplinary Sciences

WOS:001053945900001

FRONTIERS MEDIA SA

Web of Science

1.Guangzhou Univ, Precise Genome Engn Ctr, Sch Life Sci, Guangzhou, Peoples R China
2.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2,Ctr Lab Longhua Branch,Dept Infect, Shenzhen, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Shenzhen, Peoples R China
4.Naval Med Univ, Naval Med Ctr, Dept Plast Surg, Shanghai, Peoples R China

Han, Huiyu,Chen, Lianheng,Liang, Shu,et al. PLA-HPG based coating enhanced anti-biofilm and wound healing of Shikonin in MRSA-infected burn wound[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2023,11.

Han, Huiyu.,Chen, Lianheng.,Liang, Shu.,Lu, Jiawei.,Wu, Yashi.,...&Xiao, Lingyun.(2023).PLA-HPG based coating enhanced anti-biofilm and wound healing of Shikonin in MRSA-infected burn wound.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,11.

Han, Huiyu,et al."PLA-HPG based coating enhanced anti-biofilm and wound healing of Shikonin in MRSA-infected burn wound".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 11(2023).