Temporal variability of microbiome in the different plankton hosts revealed distinct environmental persistence of Vibrio parahaemolyticus in shrimp farms

Fu, Songzhe1,2; Wang, Rui2; Zhang, Jin2; Xu, Zheng3,4; Yang, Xuelin2; Yang, Qian5

2023-10-01

10.1016/j.micres.2023.127464

MICROBIOLOGICAL RESEARCH   影响因子和分区

0944-5013

1618-0623

Plankton-bacteria interactions may play essential roles in maintaining the persistence of pathogenic Vibrio spp. However, the actual plankton host of the toxigenic Vibrio parahaemolyticus that harbors thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) remains unclear. To answer this question, we measured the abundance of toxigenic and nontoxigenic Vibrio parahaemolyticus in different types of plankton by most probable number (MPN)-PCR. We next investigated the dynamics of the microbiomes of rearing water, copepods, Chlor-ella, four predominant diatom genera (Nitzschia, Melosira, Skeletonema and Chaetoceros) and the gut of the shrimp in two recirculated shrimp farming systems from April to September using high-throughput 16 S rRNA amplicon sequencing. The survival of trh-positive and trh-negative V. parahaemolyticus in seawater and different plankton hosts was examined under low temperature and starvation conditions. The results showed that copepods harbored the highest proportion of trh-positive V. parahaemolyticus, followed by diatoms. Chitinous diatoms (CD) harbored a high proportion of Vibrionaceae, of which a high abundance of V. parahaemolyticus was found in summer. In contrast, Vibrio spp. is rarely found in Chlorella and nonchitinous diatoms. Taxon-specific associations were also observed, including a relatively high abundance of Vibrio and Halodesulfovibrio on copepods and covariation of Aeromonas and Bacillus inside the Chlorella. The survival assays showed that, in comparison to trh- negative V. parahaemolyticus, trh-positive V. parahaemolyticus showed better survival in copepods and CD under starvation conditions and maintained high persistence in the above hosts at low temperature. In conclusion, the results herein suggested that chitinous plankton might provide protection for V. parahaemolyticus, especially trh- positive V. parahaemolyticus, and improve their persistence under harsh conditions. Our study provided in-depth insights into the persistence of V. parahaemolyticus in the environment, which would promote targeted disease prevention measures.

Vibrio parahaemolyticus Diatom 16 S rRNA amplicon sequencing Copepods

SCI ; EI

英语

其他

National Natural Science Foundation of China[QL202005] ; null[81903372]

Microbiology

Microbiology

WOS:001053538500001

ELSEVIER GMBH

20233214509937

Blood ; Farms ; Phytoplankton ; Plankton ; RNA ; Temperature

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Marine Science and Oceanography:471 ; Thermodynamics:641.1 ; Agricultural Equipment and Methods; Vegetation and Pest Control:821

MICROBIOLOGY

Web of Science

1.Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Xian 710069, Peoples R China
2.Dalian Ocean Univ, Key Lab Environm Controlled Aquaculture KLECA, Minist Educ, Dalian 116023, Peoples R China
3.Southern Univ Sci & Technol, Yantian Hosp, Shenzhen 518081, Peoples R China
4.Chinese Acad Sci, Inst Biomed & Biotechnol, Shenzhen Inst Adv Technol, Shenzhen 518055, Guangdong, Peoples R China
5.Univ Ghent, Ctr Microbial Ecol & Technol, B-9000 Ghent, Belgium

Fu, Songzhe,Wang, Rui,Zhang, Jin,et al. Temporal variability of microbiome in the different plankton hosts revealed distinct environmental persistence of Vibrio parahaemolyticus in shrimp farms[J]. MICROBIOLOGICAL RESEARCH,2023,275.

Fu, Songzhe,Wang, Rui,Zhang, Jin,Xu, Zheng,Yang, Xuelin,&Yang, Qian.(2023).Temporal variability of microbiome in the different plankton hosts revealed distinct environmental persistence of Vibrio parahaemolyticus in shrimp farms.MICROBIOLOGICAL RESEARCH,275.

Fu, Songzhe,et al."Temporal variability of microbiome in the different plankton hosts revealed distinct environmental persistence of Vibrio parahaemolyticus in shrimp farms".MICROBIOLOGICAL RESEARCH 275(2023).