Single-cell RNA transcriptome landscape of murine liver following systemic administration of mesoporous silica nanoparticles

Zheng, Liuhai1,2; Wu, Jiangpeng1; Hu, Hong; Cao, Hua3; Xu, Nan4; Chen, Kun1; Wen, Bowen5; Wang, Huifang1; Yuan, Haitao1; Xie, Lulin1; Jiang, Yuke1; Li, Zhifen; Liang, Cailing1; Yuan, Jimin1; Li, Zhijie1,6; Yuan, Xiaopeng1,7,8; Xiao, Wei10; Wang, Jigang1,9,10

2023-09-01

10.1016/j.jconrel.2023.07.037

JOURNAL OF CONTROLLED RELEASE   影响因子和分区

0168-3659

1873-4995

Due to the unique physicochemical properties, mesoporous silica nanoparticles (MONs) have been widely utilized in biomedical fields for drug delivery, gene therapy, disease diagnosis and imaging. With the extensive applications and large-scale production of MONs, the potential effects of MONs on human health are gaining increased attention. To better understand the cellular and molecular mechanisms underlying the effects of MONs on the mouse liver, we profiled the transcriptome of 63,783 single cells from mouse livers following weekly intravenous administration of MONs for 2 weeks. The results showed that the proportion of endothelial cells and CD4+ T cells was increased, whereas that of Kupffer cells was decreased, in a dose-dependent manner after MONs treatment in the mouse liver. We also observed that the proportion of inflammation-related Kupffer cell subtype and wound healing-related hepatocyte subtype were elevated, but the number of hepatocytes with detoxification characteristics was reduced after MONs treatment. The cell-cell communication network revealed that there was more crosstalk between cholangiocytes and Kupffer cells, liver capsular macrophages, hepatic stellate cells, and endothelial cells following MONs treatment. Furthermore, we identified key ligand-receptor pairs between crucial subtypes after MONs treatment that are known to promote liver fibrosis. Collectively, our study explored the effects of MONs on mouse liver at a single-cell level and provides comprehensive information on the potential hepatotoxicity of MONs.

MONs Transcriptome Single cells Cell-cell communication Hepatotoxicity

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[RCBS20210706092213007] ; Shenzhen Science and Technology Innovation Committee[2021A1515012164] ; National Key Clinical Specialty Construction Project, Basic and Applied Basic Research Foundation of GuangDong Province[GJHZ20200731095411034] ; International Science and Technology Cooperation for Shenzhen Technology Innovation Plan[JCYJ20210324115800001] ; null[32101219]

Chemistry ; Pharmacology & Pharmacy

Chemistry, Multidisciplinary ; Pharmacology & Pharmacy

WOS:001057965200001

ELSEVIER

PHARMACOLOGY & TOXICOLOGY

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Shenzhen Clin Res Ctr Geriatr,Dept Breast Surg,Dep, Shenzhen 518020, Guangdong, Peoples R China
2.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou 510632, Guangdong, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Oncol, Shenzhen 518020, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Gen Surg,Dept Thyroid Surg, Shenzhen 518020, Guangdong, Peoples R China
5.South China Agr Univ, Coll Nat Resources & Environm, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
6.Shanxi Datong Univ, Sch Chem & Chem Engn, Xing Yun St, Datong 037009, Shanxi, Peoples R China
7.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Lab Med, Shenzhen, Guangdong, Peoples R China
8.China Acad Chinese Med Sci, Artemisinin Res Ctr, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China
9.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
10.Guangdong Pharmaceut Univ, Key Lab Glucolipid Metab Disorder, Minist Educ, Guangzhou 510006, Guangdong, Peoples R China

Zheng, Liuhai,Wu, Jiangpeng,Hu, Hong,et al. Single-cell RNA transcriptome landscape of murine liver following systemic administration of mesoporous silica nanoparticles[J]. JOURNAL OF CONTROLLED RELEASE,2023,361.

Zheng, Liuhai.,Wu, Jiangpeng.,Hu, Hong.,Cao, Hua.,Xu, Nan.,...&Wang, Jigang.(2023).Single-cell RNA transcriptome landscape of murine liver following systemic administration of mesoporous silica nanoparticles.JOURNAL OF CONTROLLED RELEASE,361.

Zheng, Liuhai,et al."Single-cell RNA transcriptome landscape of murine liver following systemic administration of mesoporous silica nanoparticles".JOURNAL OF CONTROLLED RELEASE 361(2023).