MPP8 Governs the Activity of the LIF/STAT3 Pathway and Plays a Crucial Role in the Differentiation of Mouse Embryonic Stem Cells

Zhang, Heyao1; Yang, Tenghui1; Wu, Hao1; Yi, Wen2; Dai, Chunhong1; Chen, Xi2; Zhang, Wensheng1,3; Ye, Ying1

2023-08-01

10.3390/cells12162023

CELLS   影响因子和分区

2073-4409

Mouse embryonic stem cells (mESCs) possess the remarkable characteristics of unlimited self-renewal and pluripotency, which render them highly valuable for both fundamental research and clinical applications. A comprehensive understanding of the molecular mechanisms underlying mESC function is of the utmost importance. The Human Silence Hub (HUSH) complex, comprising FAM208A, MPP8, and periphilin, constitutes an epigenetic silencing complex involved in suppressing retroviruses and transposons during early embryonic development. However, its precise role in regulating mESC pluripotency and differentiation remains elusive. In this study, we generated homogenous miniIAA7-tagged Mpp8 mouse ES cell lines. Upon induction of MPP8 protein degradation, we observed the impaired proliferation and reduced colony formation ability of mESCs. Furthermore, this study unveils the involvement of MPP8 in regulating the activity of the LIF/STAT3 signaling pathway and Nanog expression in mESCs. Finally, we provide compelling evidence that degradation of the MPP8 protein impairs the differentiation of mESC.

mESCs MPP8 LIF/STAT3 signaling pathway Nanog differentiation

SCI

英语

其他

Cell Biology

Cell Biology

WOS:001056467000001

MDPI

Web of Science

1.Soochow Univ, Cam Su Genom Resource Ctr, Med Coll, Suzhou 215123, Peoples R China
2.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen Key Lab Gene Regulat & Syst Biol, Shenzhen 518055, Peoples R China
3.Zhejiang Univ, Zhejiang Stem & Ageing Res Z StAR Inst, Int Campus, Haining 314400, Peoples R China

Zhang, Heyao,Yang, Tenghui,Wu, Hao,et al. MPP8 Governs the Activity of the LIF/STAT3 Pathway and Plays a Crucial Role in the Differentiation of Mouse Embryonic Stem Cells[J]. CELLS,2023,12(16).

Zhang, Heyao.,Yang, Tenghui.,Wu, Hao.,Yi, Wen.,Dai, Chunhong.,...&Ye, Ying.(2023).MPP8 Governs the Activity of the LIF/STAT3 Pathway and Plays a Crucial Role in the Differentiation of Mouse Embryonic Stem Cells.CELLS,12(16).

Zhang, Heyao,et al."MPP8 Governs the Activity of the LIF/STAT3 Pathway and Plays a Crucial Role in the Differentiation of Mouse Embryonic Stem Cells".CELLS 12.16(2023).