Automated Electrowetting-based Nanobiopsy System for Adherent Cells

Muyang Ruan; Weikang Hu; Yanmei Ma; Zhen Zhan; Chengzhi Hu

10.1109/ICMA57826.2023.10215734

2023

2152-7431

979-8-3503-2085-5

2023 IEEE International Conference on Mechatronics and Automation (ICMA)

2442-2447

6-9 Aug. 2023

Harbin, Heilongjiang, China

cell nanobiopsy based on nanopipette is an effective approach to demonstrate the heterogeneity of cells and understand the roles of organelles, mRNA, and DNA of living cells. However, the manual operation of single-cell nanobiopsy has several drawbacks such as low throughput and operator variability. This paper proposes an automated nanobiopsy system containing aspiration under electrowetting based on two immiscible electrolytic solutions(TIES), cell detection, tip positioning, and contact detection for automated single-cell nanobiopsy. The physical model of TIES-based electrowetting was reported to describe the principle of aspirating fluid and evaluated to extract aqueous solution. The cell detection algorithm was utilized to obtain the nanobiopsy position of cells and evaluated on human umbilical cord vein endothelial cells (HUVEC), which provided an average of 90.8% true-positive rate (TPR) and 10.35% false-positive rate (FPR). The focus plane and tip of nanopipette were located by tip positioning algorithm based on template matching. The contact detection based on ion current feedback was employed for obtaining the distance between cells and the focus plane. Finally, the automated system accurately achieved single-cell nanobiopsy in ten to hundred living cells, which had 55.9% survival rate after sampling 220 cells totally.

Single-cell nanobiopsy electrowetting cell detection tip positioning contact detection

第一

EI

20233714732713

Automation ; Cytology ; Endothelial cells ; T-cells ; Wetting

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Immunology:461.9.1 ; Computer Software, Data Handling and Applications:723 ; Automatic Control Principles and Applications:731

IEEE

Department of Mechanical Engineering and Energy, Southern University of Science and Technology, Shenzhen, China

Muyang Ruan,Weikang Hu,Yanmei Ma,et al. Automated Electrowetting-based Nanobiopsy System for Adherent Cells[C],2023:2442-2447.