南方科技大学知识苑(SUSTech KC): Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids

题名	Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids
作者	Feng, Yilin 1,2; Zheng, Honghui 1,2; Tang, Jiyuan 1,2; Li, Xing 3; Tian, Ruilin3 ; Ma, Shaohua 1,2
通讯作者	Ma, Shaohua
发表日期	2023-09-15
DOI	10.1016/j.xpro.2023.102346
发表期刊	STAR PROTOCOLS 影响因子和分区
ISSN	2666-1667
卷号	4 期号:3
摘要	In glioma modeling, existing organoid protocols lack the ability to replicate glioma cell invasion and interaction with normal brain tissue. Here, we present a protocol for generating in vitro brain disease models using human-induced pluripotent-or embryonic-stem-cell-derived cerebral organoids (COs). We describe steps for forming glioma organoids by co-culturing forebrain organoids with U-87 MG cells. We also detail vibratome sectioning of COs to prevent cell death and enhance contact between U-87 MG cells and cerebral tissues.
相关链接	[来源记录]
收录类别	ESCI
语种	英语
学校署名	其他
资助项目	National Natural Science Foundation of China["82111530212","2021B1515020092"] ; Natural Science Foundation of Guangdong Province[SZBL2020090501 014] ; Shenzhen Bay Laboratory Fund[WDZC20 200821141349001] ; Shenzhen Science and Technology Innovation Commission["KCXFZ20200201101050887","RCYX20200714114736146","KCXFZ2020122117 3207022"] ; null[61971255]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:001058449000001
出版者	ELSEVIER
来源库	Web of Science
引用统计	被引频次[WOS]：2
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/559358
专题	南方科技大学医学院
作者单位	1.Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch SIGS, Shenzhen 518055, Peoples R China 2.Tsinghua Berkeley Shenzhen Inst TBSI, Shenzhen 518055, Peoples R China 3.Southern Univ Sci & Technol SUSTech, Sch Med, Shenzhen 518055, Peoples R China
推荐引用方式 GB/T 7714	Feng, Yilin,Zheng, Honghui,Tang, Jiyuan,et al. Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids[J]. STAR PROTOCOLS,2023,4(3).
APA	Feng, Yilin,Zheng, Honghui,Tang, Jiyuan,Li, Xing,Tian, Ruilin,&Ma, Shaohua.(2023).Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids.STAR PROTOCOLS,4(3).
MLA	Feng, Yilin,et al."Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell- derived cerebral organoids".STAR PROTOCOLS 4.3(2023).