Single-cell transcriptomic profiling reveals immune cell heterogeneity in acute myeloid leukaemia peripheral blood mononuclear cells after chemotherapy

Hu, Xuqiao1,2; Cao, Dongyan3,4; Zhou, Zhenru1,2; Wang, Zhaoyang1,2; Zeng, Jieying2; Hong, Wen-Xu1

2023-08-01

10.1007/s13402-023-00853-2

CELLULAR ONCOLOGY   影响因子和分区

2211-3428

2211-3436

PurposeAcute myeloid leukaemia (AML) is a heterogeneous disease characterised by the rapid clonal expansion of abnormally differentiated myeloid progenitor cells residing in a complex microenvironment. However, the immune cell types, status, and genome profile of the peripheral blood mononuclear cell (PBMC) microenvironment in AML patients after chemotherapy are poorly understood. In order to explore the immune microenvironment of AML patients after chemotherapy, we conducted this study for providing insights into precision medicine and immunotherapy of AML.MethodsIn this study, we used single-cell RNA sequencing (scRNA-seq) to analyse the PBMC microenvironment from five AML patients treated with different chemotherapy regimens and six healthy donors. We compared the cell compositions in AML patients and healthy donors, and performed gene set enrichment analysis (GSEA), CellPhoneDB, and copy number variation (CNV) analysis.ResultsUsing scRNA-seq technology, 91,772 high quality cells of 44,950 PBMCs from AML patients and 46,822 PBMCs from healthy donors were classified as 14 major cell clusters. Our study revealed the sub-cluster diversity of T cells, natural killer (NK) cells, monocytes, dendritic cells (DCs), and haematopoietic stem cell progenitors (HSC-Prog) in AML patients under chemotherapy. NK cells and monocyte-DCs showed significant changes in transcription factor expression and chromosome copy number variation (CNV). We also observed significant heterogeneity in CNV and intercellular interaction networks in HSC-Prog cells.ConclusionOur results elucidated the PBMC single-cell landscape and provided insights into precision medicine and immunotherapy for treating AML.

AML scRNA-seq PBMC Monocyte Chemotherapy

SCI

英语

通讯

Oncology ; Cell Biology ; Pathology

Oncology ; Cell Biology ; Pathology

WOS:001059876400001

SPRINGER

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Shenzhen Inst Dermatol, Shenzhen Ctr Chron Dis Control & Prevent, Shenzhen, Peoples R China
2.Jinan Univ, Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp,Clin Med Coll 2, Shenzhen, Peoples R China
3.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Biliary Pancreat Surg, Shanghai, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Med, Shanghai Canc Inst, State Key Lab Oncogenes & Related Genes, Shanghai, Peoples R China

Hu, Xuqiao,Cao, Dongyan,Zhou, Zhenru,et al. Single-cell transcriptomic profiling reveals immune cell heterogeneity in acute myeloid leukaemia peripheral blood mononuclear cells after chemotherapy[J]. CELLULAR ONCOLOGY,2023.

Hu, Xuqiao,Cao, Dongyan,Zhou, Zhenru,Wang, Zhaoyang,Zeng, Jieying,&Hong, Wen-Xu.(2023).Single-cell transcriptomic profiling reveals immune cell heterogeneity in acute myeloid leukaemia peripheral blood mononuclear cells after chemotherapy.CELLULAR ONCOLOGY.

Hu, Xuqiao,et al."Single-cell transcriptomic profiling reveals immune cell heterogeneity in acute myeloid leukaemia peripheral blood mononuclear cells after chemotherapy".CELLULAR ONCOLOGY (2023).