南方科技大学知识苑(SUSTech KC): Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system

题名	Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system
作者	Li，Yingnan 1; Huang，Boyu 2,3; Chen，Jian 1; Huang，Liangliang 1; Xu，Jianghai 4; Wang，Yingying 4; Cui，Guanghui 1; Zhao，Haiming 1; Xin，Beibei 1; Song，Weibin 1; Zhu，Jian Kang5,6 ; Lai，Jinsheng 1,7,8,9,10
通讯作者	Chen，Jian; Lai，Jinsheng
发表日期	2023-11-01
DOI	10.1111/pbi.14122
发表期刊	Plant Biotechnology Journal 影响因子和分区
ISSN	1467-7644
EISSN	1467-7652
卷号	21 期号:11
摘要	The CRISPR-Cas systems have been widely used as genome editing tools, with type II and V systems typically introducing small indels, and type I system mediating long-range deletions. However, the precision of type I systems for large fragment deletion is still remained to be optimized. Here, we developed a compact Cascade-Cas3 Dvu I-C system with Cas11c for plant genome editing. The Dvu I-C system was efficient to introduce controllable large fragment deletion up to at least 20 kb using paired crRNAs. The paired-crRNAs design also improved the controllability of deletions for the type I-E system. Dvu I-C system was sensitive to spacer length and mismatch, which was benefit for target specificity. In addition, we showed that the Dvu I-C system was efficient for generating stable transgenic lines in maize and rice with the editing efficiency up to 86.67%. Overall, Dvu I-C system we developed here is powerful for achieving controllable large fragment deletions.
关键词	Class I CRISPR Deletion Genome Editing Type I-C CRISPR System
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Hainan Yazhou Bay Seed Lab[B21HJ0509] ; Sanya Yazhou Bay Science and Technology City Administration[
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号	WOS:001063754500001
出版者	WILEY
ESI学科分类	PLANT & ANIMAL SCIENCE
Scopus记录号	2-s2.0-85168894315
来源库	Scopus
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/560114
专题	南方科技大学医学院_前沿生物技术研究院生命科学学院
作者单位	1.State Key Laboratory of Maize Bio-breeding,National Maize Improvement Center,Department of Plant Genetics and Breeding,China Agricultural University,Beijing,China 2.Shanghai Center for Plant Stress Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai,China 3.University of Chinese Academy of Sciences,Beijing,China 4.College of Biological Sciences,China Agricultural University,Beijing,China 5.Institute of Advanced Biotechnology and School of Life Sciences,Southern University of Science and Technology,Shenzhen,China 6.Center for Advanced Bioindustry Technologies,Chinese Academy of Agricultural Sciences,Beijing,China 7.Frontiers Science Center for Molecular Design Breeding,China Agricultural University,Beijing,China 8.Center for Crop Functional Genomics and Molecular Breeding,China Agricultural University,Beijing,China 9.Sanya Institute of China Agricultural University,Sanya,China 10.Hainan Yazhou Bay Seed Laboratory,Sanya,China
推荐引用方式 GB/T 7714	Li，Yingnan,Huang，Boyu,Chen，Jian,et al. Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system[J]. Plant Biotechnology Journal,2023,21(11).
APA	Li，Yingnan.,Huang，Boyu.,Chen，Jian.,Huang，Liangliang.,Xu，Jianghai.,...&Lai，Jinsheng.(2023).Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system.Plant Biotechnology Journal,21(11).
MLA	Li，Yingnan,et al."Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system".Plant Biotechnology Journal 21.11(2023).