南方科技大学知识苑(SUSTech KC): Genetic Code Expansion in Mammalian Cells

名称	Genetic Code Expansion in Mammalian Cells
作者	Wu，Zhigang; Wang，Jie
发布日期	2023
关键词	Cell transfection Genetic code expansion Mammalian cells Non-canonical amino acids Stable cell line
语种	英语
相关链接	[Scopus记录]
摘要	The expansion of the genetic code has enabled the incorporation of noncanonical amino acids (ncAAs) into a defined site of proteins. By introducing such a unique handle into the protein of interest (POI), bioorthogonal reactions can be utilized in live cells to monitor or manipulate the interaction, translocation, function, and modification of the POI. Here, we describe a basic protocol outlining the necessary steps to incorporate a ncAA into a POI in mammalian cells.
DOI	10.1007/978-1-0716-3251-2_11
期刊来源	Methods in Molecular Biology
卷号	2676
页码	159-167
ISSN	1064-3745
学校署名	第一 ; 通讯
Scopus记录号	2-s2.0-85160979030
来源库	Scopus
通讯作者	Wang，Jie
EISSN	1940-6029
引用统计	被引频次[WOS]：0
成果类型	其他
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/560303
专题	理学院_化学系
作者单位	Department of Chemistry,Southern University of Science and Technology,Shenzhen,China
第一作者单位	化学系
通讯作者单位	化学系
推荐引用方式 GB/T 7714	Wu，Zhigang,Wang，Jie. Genetic Code Expansion in Mammalian Cells. 2023-01-01.