Analysis of proteome and post-translational modifications of 2-hydroxyisobutyrylation reveals the glycolysis pathway in oral adenoid cystic carcinoma

Chen, Sining1,2; Li, Dandan1,3; Zeng, Zhipeng1; Zhang, Wei1; Xie, Hongliang4; Tang, Jianming4; Liao, Shengyou1; Cai, Wanxia1; Liu, Fanna2; Tang, Donge1; Dai, Yong1,5,6,7

2023-09-23

10.1186/s12957-023-03155-x

WORLD JOURNAL OF SURGICAL ONCOLOGY   影响因子和分区

1477-7819

PurposeOral adenoid cystic carcinoma (OACC) has high rates of both local-regional recurrence and distant metastasis. The objective of this study is to investigate the impact of Khib on OACC and its potential as a targeted therapeutic intervention.Experimental designWe investigated the DEPs (differentially expressed proteins) and DHMPs between OACC-T and OACC-N using LC-MS/MS-based quantitative proteomics and using several bioinformatics methods, including GO enrichment analysis, KEGG pathway analysis, subcellular localization prediction, MEA (motif enrichment analysis), and PPI (protein-protein interaction networks) to illustrate how Khib modification interfere with OACC evolution.ResultsCompared OACC-tumor samples (OACC-T) with the adjacent normal samples (OACC-N), there were 3243 of the DEPs and 2011 Khib sites were identified on 764 proteins (DHMPs). DEPs and DHMPs were strongly associated to glycolysis pathway. GAPDH of K254, ENO of K228, and PGK1 of K323 were modified by Khib in OACC-T. Khib may increase the catalytic efficiency to promote glycolysis pathway and favor OACC progression.Conclusions and clinical relevanceKhib may play a significant role in the mechanism of OACC progression by influencing the enzyme activity of the glycolysis pathway. These findings may provide new therapeutic options of OACC.

Glycolysis pathway Oral adenoid cystic carcinoma Lysine 2-hydroxyisobutyrylation Proteomics Therapeutics

SCI

英语

通讯

Oncology ; Surgery

Oncology ; Surgery

WOS:001071068400002

BMC

2-s2.0-85171897135

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Clin Med Res Ctr, Shenzhen 518020, Guangdong, Peoples R China
2.Jinan Univ, Affiliated Hosp 1, Nephrol Dept, Guangzhou 510632, Peoples R China
3.Shenzhen Pingle Orthoped Hosp, Shenzhen Pingshan Tradit Chinese Med Hosp, Expt Ctr, Shenzhen 518118, Guangdong, Peoples R China
4.Jinan Univ, Dept Oral & Maxillofacial Surg, Shenzhen Peoples Hosp, Clin Med Coll 2,Stomatol Med Ctr, Shenzhen 518020, Peoples R China
5.Southern Univ Sci & Technol, Taizhou Res Inst, Comprehens Hlth Ind Res Ctr, Taizhou 318000, Peoples R China
6.924 Hosp PLA Joint Logist Support Force, Dept Organ Transplantat, Med Qual Specialty Joint Logist Support Force, Guilin 541002, Peoples R China
7.Anhui Univ Sci & Technol, Affiliated Hosp 1, Sch Med, Huainan 232001, Anhui, Peoples R China

Chen, Sining,Li, Dandan,Zeng, Zhipeng,et al. Analysis of proteome and post-translational modifications of 2-hydroxyisobutyrylation reveals the glycolysis pathway in oral adenoid cystic carcinoma[J]. WORLD JOURNAL OF SURGICAL ONCOLOGY,2023,21(1).

Chen, Sining.,Li, Dandan.,Zeng, Zhipeng.,Zhang, Wei.,Xie, Hongliang.,...&Dai, Yong.(2023).Analysis of proteome and post-translational modifications of 2-hydroxyisobutyrylation reveals the glycolysis pathway in oral adenoid cystic carcinoma.WORLD JOURNAL OF SURGICAL ONCOLOGY,21(1).

Chen, Sining,et al."Analysis of proteome and post-translational modifications of 2-hydroxyisobutyrylation reveals the glycolysis pathway in oral adenoid cystic carcinoma".WORLD JOURNAL OF SURGICAL ONCOLOGY 21.1(2023).