LIF Aggravates Pulpitis by Promoting Inflammatory Response in Macrophages

Guo, Donghua1; Dong, Wei1; Cong, Yaqi1; Liu, Yi2; Liang, Youde3; Ye, Zhou4; Zhang, Jiali1; Zhou, Yi1,5

2023-10-01

10.1007/s10753-023-01910-6

INFLAMMATION   影响因子和分区

0360-3997

1573-2576

Leukemia inhibitory factor (LIF) has been recognized as a novel inflammatory modulator in inflammation-associated diseases. This study aimed to investigate the modulation of LIF in dental pulp inflammation. Experimental pulpitis was established in wild-type (WT) and Lif-deficient (Lif-/-) mice. Histological and immunostaining analyses were conducted to assess the role of LIF in the progression of pulpitis. Mouse macrophage cell line (RAW264.7) was treated with LPS to simulate an inflammatory environment. Exogenous LIF was added to this system to examine its modulation in macrophage inflammatory response in vitro. Primary bone marrow-derived macrophages (BMDMs) from WT and Lif-/- mice were isolated and stimulated with LPS to confirm the effect of Lif deletion on macrophage inflammatory response. Supernatants from LIF and LPS-treated human dental pulp cells (hDPCs) were collected and added to macrophages. Macrophage chemotaxis was assessed using transwell assays. The results showed an increased expression of LIF and LIFR with the progression of pulpitis, and LIFR was highly expressed in macrophages. Lif deficiency alleviated experimental pulpitis with the reduction of pro-inflammatory cytokines and macrophage infiltration. Exogenous LIF promoted inflammatory response of LPS-induced macrophages through a STAT3/p65-dependent pathway. Consistently, Lif deletion inhibited macrophage inflammatory response in vitro. Supernatants of LIF-treated hDPCs enhanced macrophage migration in LPS-induced inflammatory environment. Our findings demonstrated that LIF aggravates pulpitis by promoting macrophage inflammatory response through a STAT3/p65-dependent pathway. Furthermore, LIF plays a crucial role in driving the recruitment of macrophages to inflamed pulp tissue by promoting chemokine secretion in DPCs.

Leukemia inhibitory factor Macrophage Inflammation Pulpitis

SCI

英语

其他

Cell Biology ; Immunology

Cell Biology ; Immunology

WOS:001073667400001

SPRINGER/PLENUM PUBLISHERS

IMMUNOLOGY

2-s2.0-85173077971

Web of Science

1.Wuhan Univ, Key Lab Oral Biomed, Hubei Key Lab Stomatol, State Key Lab Oral & Maxillofacial Reconstruct & R, Wuhan 430079, Peoples R China
2.Hubei Polytech Univ, Huangshi Cent Hosp, Affiliated Hosp, Edong Healthcare Grp,Dept Stomatol, Huangshi, Peoples R China
3.Southern Univ Sci & Technol, Yantian Hosp, Shenzhen, Peoples R China
4.Univ Hong Kong, Fac Dent, Appl Oral Sci & Community Dent Care, Hong Kong, Peoples R China
5.Wuhan Univ, Sch & Hosp Stomatol, Ctr Orthodont & Pediat Dent, Opt Valley Branch, Wuhan, Peoples R China

Guo, Donghua,Dong, Wei,Cong, Yaqi,et al. LIF Aggravates Pulpitis by Promoting Inflammatory Response in Macrophages[J]. INFLAMMATION,2023,47(1):307-322.

Guo, Donghua.,Dong, Wei.,Cong, Yaqi.,Liu, Yi.,Liang, Youde.,...&Zhou, Yi.(2023).LIF Aggravates Pulpitis by Promoting Inflammatory Response in Macrophages.INFLAMMATION,47(1),307-322.

Guo, Donghua,et al."LIF Aggravates Pulpitis by Promoting Inflammatory Response in Macrophages".INFLAMMATION 47.1(2023):307-322.