Super-Resolution Mapping of Single Nanoparticles inside Tumor Spheroids

Liu，Yongtao1; Wang，Fan1; Lu，Hongxu2; Fang，Guocheng1; Wen，Shihui1; Chen，Chaohao1; Shan，Xuchen1; Xu，Xiaoxue1; Zhang，Lin2; Stenzel，Martina2; Jin，Dayong1,3

2020-02-01

10.1002/smll.201905572

Small   影响因子和分区

1613-6810

1613-6829

Cancer spheroids have structural, functional, and physiological similarities to the tumor, and have become a low-cost in vitro model to study the physiological responses of single cells and therapeutic efficacy of drugs. However, the tiny spheroid, made of a cluster of high-density cells, is highly scattering and absorptive, which prevents light microscopy techniques to reach the depth inside spheroids with high resolution. Here, a method is reported for super-resolution mapping of single nanoparticles inside a spheroid. It first takes advantage of the self-healing property of a “nondiffractive” doughnut-shaped Bessel beam from a 980 nm diode laser as the excitation, and further employs the nonlinear response of the 800 nm emission from upconversion nanoparticles, so that both excitation and emission at the near-infrared can experience minimal loss through the spheroid. These strategies lead to the development of a new nanoscopy modality with a resolution of 37 nm, 1/26th of the excitation wavelength. This method enables mapping of single nanoparticles located 55 µm inside a spheroid, with a resolution of 98 nm. It suggests a solution to track single nanoparticles and monitor their release of drugs in 3D multicellar environments.

deep tissue super-resolution tumor spheroids upconversion nanoparticles (UCNPs)

SCI ; EI

英语

其他

National Natural Science Foundation of China[51720105015] ; China Scholarship Council[201708200004] ; University of Technology Sydney[PRO17-4393] ; [ACSRF65827] ; [KQTD20170810110913065]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000506932500001

Wiley-VCH Verlag

20200308051588

Infrared devices ; Laser excitation ; Mapping ; Nanoparticles ; Optical resolving power ; Physiological models ; Physiology ; Tumors

Surveying:405.3 ; Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Light/Optics:741.1 ; Laser Applications:744.9 ; Nanotechnology:761 ; Solid State Physics:933

2-s2.0-85077848165

Scopus

1.Institute for Biomedical Materials and Devices (IBMD)/Faculty of Science,University of Technology Sydney,Sydney,2007,Australia
2.School of Chemistry/Centre for Advanced Macromolecular Design (CAMD),University of New South Wales,Sydney,Kensington,2052,Australia
3.UTS-SUStech Joint Research Centre for Biomedical Materials & Devices,Southern University of Science and Technology,Guangdong,Shenzhen,518055,China

Liu，Yongtao,Wang，Fan,Lu，Hongxu,et al. Super-Resolution Mapping of Single Nanoparticles inside Tumor Spheroids[J]. Small,2020,16(6).

Liu，Yongtao.,Wang，Fan.,Lu，Hongxu.,Fang，Guocheng.,Wen，Shihui.,...&Jin，Dayong.(2020).Super-Resolution Mapping of Single Nanoparticles inside Tumor Spheroids.Small,16(6).

Liu，Yongtao,et al."Super-Resolution Mapping of Single Nanoparticles inside Tumor Spheroids".Small 16.6(2020).