Electrochemical Monitoring of Intracellular Reactive Oxygen Species Based on Automated Nanoprobe Platform

Yanmei Ma1; Weikang Hu2; Muyang Ruan1; Zhen Zhan1; Yi Zhang1; Chengzhi Hu1

10.1109/3M-NANO58613.2023.10305373

2023-07-31

2373-5422

979-8-3503-4449-3

2023 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

443-447

31 July-4 Aug. 2023

Chengdu, China

The electrochemical detection of endogenous molecules from a single cell is a crucial technique for understanding fundamental cellular processes at the subcellular level and uncovering cellular heterogeneity. However, high throughput intracellular detection has posed significant challenges. This paper proposes a simple method for fabricating a nanoprobe for the electrochemical monitoring of intracellular reactive oxygen species (ROS), which are important signaling molecules that play a key role in a wide range of cellular processes, including apoptosis, proliferation, and differentiation. Combined with the automated nanoprobe platform, the study successfully achieved minimally invasive and efficient intracellular ROS detection in living cells. Moreover, the results reveal that the average ROS concentration in the cytoplasm and nucleus is uneven, which has important implications for the understanding of cellular redox signaling.

relative oxygen species single-cell detection automated nanoprobe platform

第一

EI

20235015191449

Automation ; Cell death ; Chemical detection ; Molecules ; Oxygen

Biology:461.9 ; Automatic Control Principles and Applications:731 ; Optical Devices and Systems:741.3 ; Nanotechnology:761 ; Chemistry:801 ; Chemical Products Generally:804 ; Atomic and Molecular Physics:931.3 ; Solid State Physics:933

IEEE

1.Department of Mechanical and Energy Engineering, Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems, Southern University of Science and Technology, Shenzhen, China
2.Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China

Yanmei Ma,Weikang Hu,Muyang Ruan,et al. Electrochemical Monitoring of Intracellular Reactive Oxygen Species Based on Automated Nanoprobe Platform[C],2023:443-447.