Droplet-based Microfluidic Platform for High-throughput Formation of Multicellular Spheroids

Zhen Zhan; Hanhan Xie; Chengzhi Hu

10.1109/3M-NANO58613.2023.10305351

2023-07-31

2373-5422

979-8-3503-4449-3

2023 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)

238-243

31 July-4 Aug. 2023

Chengdu, China

Cell culture in vitro is essential for drug screening, disease modeling, and stem cell-related research. Previous studies have indicated that two-dimensional (2D) cellular environments can significantly differ from physiological cells in vivo, leading to a high failure rate when translating therapeutics developed in 2D cell models to clinical applications. Therefore, there is a strong focus on developing three-dimensional (3D) cell culture techniques that more closely mimic in vivo cell microenvironments. These techniques hold great promise for enhancing precision in drug discovery and tissue regeneration. In this study, we introduce a novel microfluidic technique that utilizes droplets to fabricate hydrogel microspheres, each exhibiting a unique internal morphology suitable for 3D cell culture applications. Specifically, we employed this method to cultivate human umbilical vein endothelial cells (HUVECs) within the interior canals of the hydrogel microspheres, forming monolayers on the surfaces of the spiral canals. This setup effectively mimicked the configurations of native blood vessels. Furthermore, we successfully demonstrated the fabrication of heterogeneous spheroids with spiral blood vessel lumens, which can be utilized to construct spiral vascularized bone tissue.

microfluidics tissue engineering multicellular spheroids coculture hydrogel

第一

EI

20235015191428

Blood ; Blood vessels ; Bone ; Canals ; Cell culture ; Diagnosis ; Drops ; Endothelial cells ; Hydraulic structures ; Hydrogels ; Microspheres ; Stem cells

Waterways:407.2 ; Waterway Transportation, General:434.1 ; Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Microfluidics:632.5.1 ; Colloid Chemistry:801.3 ; Chemical Products Generally:804

IEEE

Department of Mechanical and Energy Engineering, Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems, Southern University of Science and Technology, Shenzhen, China

Zhen Zhan,Hanhan Xie,Chengzhi Hu. Droplet-based Microfluidic Platform for High-throughput Formation of Multicellular Spheroids[C],2023:238-243.