南方科技大学知识苑(SUSTech KC): Radix Bupleuri-Radix Paeoniae Alba Inhibits the Development of Hepatocellular Carcinoma through Activation of the PTEN/PD-L1 Axis within the Immune Microenvironment

题名	Radix Bupleuri-Radix Paeoniae Alba Inhibits the Development of Hepatocellular Carcinoma through Activation of the PTEN/PD-L1 Axis within the Immune Microenvironment
作者	Zhang, Fan 1 ; Zhou, Kun 2; Yuan, Wei 3; Sun, Kewei 3,4
通讯作者	Sun, Kewei
发表日期	2023-10-01
DOI	10.1080/01635581.2023.2276525
发表期刊	NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL 影响因子和分区
ISSN	0163-5581
EISSN	1532-7914
摘要	ObjectiveThis study investigated how Radix Bupleuri-Radix Paeoniae Alba (BP) was active against hepatocellular carcinoma (HCC).MethodsTraditional Chinese medicine systems pharmacology (TCMSP) database was employed to determine the active ingredients of BP and potential targets against HCC. Molecular docking analysis verified the binding activity of PTEN with BP ingredients. H22 cells were used to establish an HCC model in male balb/c mice. Immunofluorescence staining, immunohistochemistry, flow cytometry, western blotting, enzyme-linked immunosorbent assay, and real-time quantitative PCR were used to study changes in proliferation, apoptosis, PTEN levels, inflammation, and T-cell differentiation in male balb/c mice.ResultsThe major active ingredients in BP were found to be quercetin, kaempferol, isorhamnetin, stigmasterol, and beta-sitosterol. Molecular docking demonstrated that these five active BP ingredients formed a stable complex with PTEN. BP exhibited an anti-tumor effect in our HCC mouse model. BP was found to increase the CD8+ and IFN-gamma+/CD4+ T cell levels while decreasing the PD-1+/CD8+ T and Treg cell levels in HCC mice. BP up-regulated the IL-6, IFN-gamma, and TNF-alpha levels but down-regulated the IL-10 levels in HCC mice. After PTEN knockdown, BP-induced effects were abrogated.ConclusionBP influenced the immune microenvironment through activation of the PTEN/PD-L1 axis, protecting against HCC.
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一
资助项目	Shenzhen Science and Technology Innovation Fund ([2022])["47","JCYJ20220530163407018"] ; Shenzhen Third People's Hospital, The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Research on Internal Topics[22240G1045]
WOS研究方向	Oncology ; Nutrition & Dietetics
WOS类目	Oncology ; Nutrition & Dietetics
WOS记录号	WOS:001092310900001
出版者	ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD
ESI学科分类	CLINICAL MEDICINE
来源库	Web of Science
引用统计	被引频次[WOS]：4
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/582791
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Dept TCM, Affiliated Hosp 2, Shenzhen, Guangdong, Peoples R China 2.Guangzhou Univ Chinese Med, Dept Hepatol, Shenzhen Hosp, Shenzhen, Guangdong, Peoples R China 3.Hunan Univ Tradit Chinese Med, Dept Hepatol, Affiliated Hosp 1, Changsha, Hunan, Peoples R China 4.Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, 113 Middle Shaoshan Rd, Changsha 410007, Hunan, Peoples R China
第一作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Zhang, Fan,Zhou, Kun,Yuan, Wei,et al. Radix Bupleuri-Radix Paeoniae Alba Inhibits the Development of Hepatocellular Carcinoma through Activation of the PTEN/PD-L1 Axis within the Immune Microenvironment[J]. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL,2023.
APA	Zhang, Fan,Zhou, Kun,Yuan, Wei,&Sun, Kewei.(2023).Radix Bupleuri-Radix Paeoniae Alba Inhibits the Development of Hepatocellular Carcinoma through Activation of the PTEN/PD-L1 Axis within the Immune Microenvironment.NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL.
MLA	Zhang, Fan,et al."Radix Bupleuri-Radix Paeoniae Alba Inhibits the Development of Hepatocellular Carcinoma through Activation of the PTEN/PD-L1 Axis within the Immune Microenvironment".NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL (2023).