南方科技大学知识苑(SUSTech KC): TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype

题名	TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype
作者	Karanovic, Una 1; Lei, Lei 1; Martens, Craig A.2; Barbian, Kent 2; Mcclarty, Grant 3; Caldwell, Harlan D.1; Yang, Chunfu4
通讯作者	Yang, Chunfu
发表日期	2023-01-17
DOI	10.1093/femspd/ftad026
发表期刊	PATHOGENS AND DISEASE 影响因子和分区
ISSN	2049-632X
卷号	81
摘要	Chlamydia trachomatis is an obligate intracellular bacterium that causes blinding trachoma and sexually transmitted disease. The chlamydial plasmid is a critical virulence factor in the pathogenesis of these diseases. Plasmid gene protein 4 (Pgp4) plays a major role in chlamydial virulence by regulating the expression of both chromosomal genes and Pgp3. Despite the importance of Pgp4 in mediating lytic exit from host cells the pathogenic mechanism by which it functions is unknown. CT084 is a highly conserved chromosomal gene with homology to phospholipase D. We showed CT084 expression is regulated by Pgp4 and expressed late in the chlamydial developmental cycle. To investigate the function of CT084 in chlamydial lytic exit from infected cells, we made a CT084 null strain (ct084::bla) by using Targetron. The ct084::bla strain grew normally in vitro compared to wild-type strain; however, the strain did not lyse infected cells and produced significantly less and smaller plaques. Collectively, our finding shows Pgp4-regulated CT084-mediated chlamydia lytic exit from infected host cells.
关键词	Chlamydia trachomatis plasmid lytic exit phospholipase CT084
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	National Natural Science Foundation of China[32270203]
WOS研究方向	Immunology ; Infectious Diseases ; Microbiology
WOS类目	Immunology ; Infectious Diseases ; Microbiology
WOS记录号	WOS:001087241800001
出版者	OXFORD UNIV PRESS
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/582844
专题	南方科技大学医学院_公共卫生及应急管理学院
作者单位	1.NIAID, Lab Clin Immunol & Microbiol, NIH, Bethesda, MD USA 2.NIAID, Rocky Mt Labs, Hamilton, MT 59840 USA 3.Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB R3T 2N2, Canada 4.Southern Univ Sci & Technol, Sch Publ Hlth & Emergency Management, Room 403,7th Bldg,Chuang Yuan 1088,Xueyuan Ave, Shenzhen 518055, Guangdong, Peoples R China
通讯作者单位	公共卫生及应急管理学院
推荐引用方式 GB/T 7714	Karanovic, Una,Lei, Lei,Martens, Craig A.,et al. TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype[J]. PATHOGENS AND DISEASE,2023,81.
APA	Karanovic, Una.,Lei, Lei.,Martens, Craig A..,Barbian, Kent.,Mcclarty, Grant.,...&Yang, Chunfu.(2023).TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype.PATHOGENS AND DISEASE,81.
MLA	Karanovic, Una,et al."TargeTron inactivation of plasmid-regulated Chlamydia trachomatis CT084 results in a nonlytic phenotype".PATHOGENS AND DISEASE 81(2023).