南方科技大学知识苑(SUSTech KC): BRD9-mediated control of the TGF-Î²/Activin/Nodal pathway regulates self-renewal and differentiation of human embryonic stem cells and progression of cancer cells

题名	BRD9-mediated control of the TGF-Î²/Activin/Nodal pathway regulates self-renewal and differentiation of human embryonic stem cells and progression of cancer cells
作者	Wang, Xuepeng 1; Song, Chengcheng 2; Ye, Ying 3; Gu, Yashi 4; Li, Xuemei 5; Chen, Peixin 3; Leng, Dongliang 2; Xiao, Jing 2; Wu, Hao 3; Xie, Sisi 4; Liu, Weiwei 2; Zhao, Qi ; Chen, Di 4; Chen, Xi6 ; Wu, Qiang 1,7; Chen, Guokai 2; Zhang, Wensheng 3,5,8
通讯作者	Wu, Qiang; Chen, Guokai; Zhang, Wensheng
发表日期	2023-10-01
DOI	10.1093/nar/gkad907
发表期刊	NUCLEIC ACIDS RESEARCH 影响因子和分区
ISSN	0305-1048
EISSN	1362-4962
摘要	["Bromodomain-containing protein 9 (BRD9) is a specific subunit of the non-canonical SWI/SNF (ncBAF) chromatin-remodeling complex, whose function in human embryonic stem cells (hESCs) remains unclear. Here, we demonstrate that impaired BRD9 function reduces the self-renewal capacity of hESCs and alters their differentiation potential. Specifically, BRD9 depletion inhibits meso-endoderm differentiation while promoting neural ectoderm differentiation. Notably, supplementation of NODAL, TGF-beta, Activin A or WNT3A rescues the differentiation defects caused by BRD9 loss. Mechanistically, BRD9 forms a complex with BRD4, SMAD2/3, beta-CATENIN and P300, which regulates the expression of pluripotency genes and the activity of TGF-beta/Nodal/Activin and Wnt signaling pathways. This is achieved by regulating the deposition of H3K27ac on associated genes, thus maintaining and directing hESC differentiation. BRD9-mediated regulation of the TGF-beta/Activin/Nodal pathway is also demonstrated in the development of pancreatic and breast cancer cells. In summary, our study highlights the crucial role of BRD9 in the regulation of hESC self-renewal and differentiation, as well as its participation in the progression of pancreatic and breast cancers.","Graphical Abstract"]
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:001087681100001
出版者	OXFORD UNIV PRESS
ESI学科分类	BIOLOGY & BIOCHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/582850
专题	生命科学学院_生物系生命科学学院
作者单位	1.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa 999078, Macao, Peoples R China 2.Univ Macau, Fac Hlth Sci, Ctr Reprod Dev & Aging, Taipa 999078, Macao, Peoples R China 3.Soochow Univ, Med Coll, Suzhou 215123, Peoples R China 4.Zhejiang Univ, Zhejiang Univ Univ Edinburgh Inst ZJE, Sch Med, Haining 314400, Peoples R China 5.Binzhou Med Univ, Peninsula Canc Res Ctr, Sch Basic Med Sci, Yantai 264003, Peoples R China 6.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518000, Peoples R China 7.Macau Univ Sci & Technol, Precis Regenerat Med Ctr, Taipa 999078, Macao, Peoples R China 8.Shandong Univ Technol, Sch Life Sci & Med, Zibo 255049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xuepeng,Song, Chengcheng,Ye, Ying,et al. BRD9-mediated control of the TGF-Î²/Activin/Nodal pathway regulates self-renewal and differentiation of human embryonic stem cells and progression of cancer cells[J]. NUCLEIC ACIDS RESEARCH,2023.
APA	Wang, Xuepeng.,Song, Chengcheng.,Ye, Ying.,Gu, Yashi.,Li, Xuemei.,...&Zhang, Wensheng.(2023).BRD9-mediated control of the TGF-Î²/Activin/Nodal pathway regulates self-renewal and differentiation of human embryonic stem cells and progression of cancer cells.NUCLEIC ACIDS RESEARCH.
MLA	Wang, Xuepeng,et al."BRD9-mediated control of the TGF-Î²/Activin/Nodal pathway regulates self-renewal and differentiation of human embryonic stem cells and progression of cancer cells".NUCLEIC ACIDS RESEARCH (2023).