Genome editing in rice using CRISPR/Cas12i3

Lv, Ping1,2; Su, Fei1,2,3; Chen, Fangyuan1,2; Yan, Chunxue1,2; Xia, Dandan1,2; Sun, Hui1; Li, Shanshan1; Duan, Zhiqiang1; Ma, Changle2; Zhang, Hui4; Wang, Mugui5; Niu, Xiaomu1; Zhu, Jian-Kang3,6,7; Zhang, Jinshan1,2

2023-10-01

10.1111/pbi.14192

PLANT BIOTECHNOLOGY JOURNAL   影响因子和分区

1467-7644

1467-7652

The CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide-resistant rice plants using the base editors. These CRIPSR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.

CRISPR/Cas12i3 Multiplex DR-spacer Array DNA structural variations Base editors

SCI

英语

通讯

This work was supported by the Key Research and Development Program of Shandong Province (Agricultural Seed Improvement Project, 2022LZGC001).[2022LZGC001]

Biotechnology & Applied Microbiology ; Plant Sciences

Biotechnology & Applied Microbiology ; Plant Sciences

WOS:001083191500001

WILEY

PLANT & ANIMAL SCIENCE

2-s2.0-85173891992

Web of Science

1.Bellagen Biotechnol Co Ltd, Jinan, Peoples R China
2.Qilu Normal Univ, Sch Life Sci, Jinan, Peoples R China
3.Chinese Acad Agr Sci, Ctr Adv Bioind Technol, Beijing, Peoples R China
4.Shanghai Normal Univ, Coll Life Sci, Shanghai, Peoples R China
5.Chinese Acad Agr Sci, Natl Nanfan Res Inst Sanya, Sanya, Peoples R China
6.Southern Univ Sci & Technol, Inst Adv Biotechnol, Shenzhen, Peoples R China
7.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen, Peoples R China

Lv, Ping,Su, Fei,Chen, Fangyuan,et al. Genome editing in rice using CRISPR/Cas12i3[J]. PLANT BIOTECHNOLOGY JOURNAL,2023,22(2):379-385.

Lv, Ping.,Su, Fei.,Chen, Fangyuan.,Yan, Chunxue.,Xia, Dandan.,...&Zhang, Jinshan.(2023).Genome editing in rice using CRISPR/Cas12i3.PLANT BIOTECHNOLOGY JOURNAL,22(2),379-385.

Lv, Ping,et al."Genome editing in rice using CRISPR/Cas12i3".PLANT BIOTECHNOLOGY JOURNAL 22.2(2023):379-385.