c-Jun as a one-way valve at the naive to primed interface

Li, Dongwei1,3; Luo, Ling3,4; Guo, Lin3; Wu, Chuman3; Zhang, Ran2,4; Peng, Yuling1; Wu, Menghua1; Kuang, Junqi3; Li, Yan3; Zhang, Yudan3; Xie, Jun4; Xie, Wenxiu3; Mao, Rui3; Ma, Gang5; Fu, Xiuling5; Chen, Jiekai3,4; Hutchins, Andrew P.5; Pei, Duanqing6

2023-10-14

10.1186/s13578-023-01141-0

CELL AND BIOSCIENCE   影响因子和分区

2045-3701

Backgroundc-Jun is a proto-oncogene functioning as a transcription factor to activate gene expression under many physiological and pathological conditions, particularly in somatic cells. However, its role in early embryonic development remains unknown.ResultsHere, we show that c-Jun acts as a one-way valve to preserve the primed state and impair reversion to the naive state. c-Jun is induced during the naive to primed transition, and it works to stabilize the chromatin structure and inhibit the reverse transition. Loss of c-Jun has surprisingly little effect on the naive to primed transition, and no phenotypic effect on primed cells, however, in primed cells the loss of c-Jun leads to a failure to correctly close naive-specific enhancers. When the primed cells are induced to reprogram to a naive state, these enhancers are more rapidly activated when c-Jun is lost or impaired, and the conversion is more efficient.ConclusionsThe results of this study indicate that c-Jun can function as a chromatin stabilizer in primed EpiSCs, to maintain the epigenetic cell type state and act as a one-way valve for cell fate conversions.

EpiSCs c-Jun Naive to primed transition Primed to naive transition

SCI

英语

其他

This work was supported in part by the National Natural Science Foundation of China (32270574, 31830060, 92068201, 81570279, 81974019, 32150710521), Major State Basic Research Development Program (2017YFA0504100), National Key Research and Development Prog["32270574","31830060","92068201","81570279","81974019","32150710521"] ; National Natural Science Foundation of China[2017YFA0504100] ; Major State Basic Research Development Program["2018YFA0108700","2017YFA0105602"] ; National Key Research and Development Program of China[81720108004] ; NSFC Projects of International Cooperation and Exchanges["2019348","2023A03J0045"] ; Youth Innovation Promotion of the Chinese Academy of Sciences[2020XXG002] ; Guangdong Cardiovascular Institute["DFJHBF202110","DFJHBF202111"]

Biochemistry & Molecular Biology

Biochemistry & Molecular Biology

WOS:001084294900001

BMC

2-s2.0-85174290505

Web of Science

1.Guangzhou Med Univ, Therapy & Rehabil Guangdong Higher Educ Inst, Key Lab Biol Targeting Diag, Affiliated Hosp 5, 190 Kaiyuan Dadao, Guangzhou 510799, Peoples R China
2.Southern Med Univ, Guangdong Prov Peoples Hosp, Guangdong Cardiovasc Inst, Guangdong Acad Med Sci,Dept Guangdong Prov Key Lab, Guangzhou 510100, Guangdong, Peoples R China
3.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Ctr Cell Lineage & Dev, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou 510530, Peoples R China
4.Guangzhou Regenerat Med & Hlth Guangdong Lab, Bioland Lab, Guangzhou 510005, Peoples R China
5.Southern Univ Sci & Technol, Sch Life Sci, Dept Syst Biol, Shenzhen 518055, Peoples R China
6.Westlake Univ, Sch Life Sci, Lab Cell Fate Control, 18 Longshan St, Hangzhou 310024, Peoples R China

Li, Dongwei,Luo, Ling,Guo, Lin,et al. c-Jun as a one-way valve at the naive to primed interface[J]. CELL AND BIOSCIENCE,2023,13(1).

Li, Dongwei.,Luo, Ling.,Guo, Lin.,Wu, Chuman.,Zhang, Ran.,...&Pei, Duanqing.(2023).c-Jun as a one-way valve at the naive to primed interface.CELL AND BIOSCIENCE,13(1).

Li, Dongwei,et al."c-Jun as a one-way valve at the naive to primed interface".CELL AND BIOSCIENCE 13.1(2023).