Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads

Chai, Fengli1; Wang, Dou1; Shi, Fei2; Zheng, Wenfu3; Zhao, Xiaohui1; Chen, Yao1; Mao, Cuiping1; Zhang, Jiangjiang1,4; Jiang, Xingyu1

2023-09-22

10.1021/acs.nanolett.3c02828

NANO LETTERS   影响因子和分区

1530-6984

1530-6992

Sepsis is an acute systemic infectious syndrome with high fatality. Fast and accurate diagnosis, monitoring, and medication of sepsis are essential. We exploited the fluorescent metal-AIEgen frameworks (MAFs) and demonstrated the dual functions of protein detection and bacteria identification: (i) ultrasensitive point-of-care (POC) detection of sepsis biomarkers (100 times enhanced sensitivity); (ii) rapid POC identification of Gram-negative/positive bacteria (selective aggregation within 20 min). Fluorescent lateral flow immunoassays (LFAs) are convenient and inexpensive for POC tests. MAFs possess a large surface area, excellent photostability, high quantum yield (similar to 80%), and multiple active sites serving as protein binding domains for ultrasensitive detection of sepsis biomarkers (IL-6/PCT) on LFAs. The limit of detection (LOD) for IL-6/PCT is 0.252/0.333 pg/mL. Rapid appraisal of infectious bacteria is vital to guide the use of medicines. The dual-functional fluorescent MAFs have great potential in POC tests for the clinical diagnosis of bacterial infections.

Sepsis diagnosis Inflammation factor detections Lateral flow immunoassays Bacterial recognition Metal-organic frameworks

SCI ; EI

英语

NI论文

第一 ; 通讯

National Key R&D Program of China["2021YFF1200800","2022YFF1202900","2018YFA0902600"] ; National Natural Science Foundation of China["22104049","22234004","22204068","32071390"] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003] ; Shenzhen Science and Technology Program["KQTD20190929172743294","JCYJ20210324105006017","KCXFZ20211020163544002"] ; Guangdong Major Talent Introduction Project[2019CX01Y196] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:001071388800001

AMER CHEMICAL SOC

20234414998590

Bacteria ; Binding sites ; Biomarkers ; Fluorescence ; Organometallics ; Proteins

Medicine and Pharmacology:461.6 ; Light/Optics:741.1 ; Biochemistry:801.2 ; Organic Compounds:804.1

MATERIALS SCIENCE

Web of Science

1.Southern Univ Sci & Technol, Shenzhen Key Lab Smart Healthcare Engn, Guangdong Prov Key Lab Adv Biomat, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
2.Jinan Univ, Clin Med Coll 2, Southern Univ Sci & Technol, Shenzhen Peoples Hosp,Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
3.Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China
4.Beijing Inst Technol, Sch Life Sci, Minist Ind & Informat Technol, Key Lab Mol Med & Biotherapy, Beijing 100081, Peoples R China

Chai, Fengli,Wang, Dou,Shi, Fei,et al. Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads[J]. NANO LETTERS,2023,23(19):9056-9064.

Chai, Fengli.,Wang, Dou.,Shi, Fei.,Zheng, Wenfu.,Zhao, Xiaohui.,...&Jiang, Xingyu.(2023).Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads.NANO LETTERS,23(19),9056-9064.

Chai, Fengli,et al."Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads".NANO LETTERS 23.19(2023):9056-9064.