Superoxide dismutase A (SodA) is a c-di-GMP effector protein governing oxidative stress tolerance in Stenotrophomonas maltophilia

Sun，Xiao Yu1,2,3; Deng，Jie1,7; Zhang，Chenhui7; Fung，Sin Yee2; Siu，Kam Leung2; Cheng，Ying Ying4,5; Ye，Liumei6; Qin，Jiaoxia6; Wang，Ke6; Qu，Jiu Xin7; Gao，Wenying7; Wang，Fuxiang7; Jin，Dong Yan2; Yang，Liang1,7

2024

10.1016/j.micres.2023.127535

Microbiological Research   影响因子和分区

0944-5013

C-di-GMP is a bacterial second messenger implicated in the regulation of many key functions including antibiotic tolerance and biofilm formation. Our understanding of how c-di-GMP exerts its action via receptors to modulate different biological functions is still limited. Here we used a c-di-GMP affinity pull-down assay coupled to LC-MS/MS to identify c-di-GMP-binding proteins in the opportunistic pathogen Stenotrophomonas maltophilia. This analysis identified Smlt3238 (SodA), a protein of the superoxide dismutase family, as a c-di-GMP-binding protein. Microscale thermophoresis showed that purified SodA protein bound c-di-GMP with an estimated dissociation constant (Kd) value of 141.5 μM. Using various in vivo and in vitro experiments, we demonstrated that c-di-GMP modulates the enzyme activity of SodA directly. Circular dichroism experiments revealed that SodA protein gradually altered its basic structure with increasing levels of c-di-GMP. Phenotypic experiments conducted in the presence of a range of intracellular c-di-GMP levels showed that SodA function is modulated by c-di-GMP. The findings thus identify a novel c-di-GMP binding protein that governs oxidative stress tolerance in S. maltophilia.

Anti-oxidation C-di-GMP Oxidative stress Superoxide dismutase

SCI ; EI

英语

第一

MICROBIOLOGY

2-s2.0-85175318389

Scopus

1.School of Medicine,Southern University of Science and Technology / Shenzhen Key Laboratory for Gene Regulation and Systems Biology,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
2.School of Biomedical Sciences,The University of Hong Kong,Pokfulam,21 Sassoon Road,Hong Kong
3.Tianjin Key Laboratory of Retinal Functions and Diseases,Tianjin Branch of National Clinical Research Center for Ocular Disease,Eye Institute and School of Optometry,Tianjin Medical University Eye Hospital,Tianjin,China
4.Shenzhen Institute of Respiratory Diseases,Second Clinical Medical College (Shenzhen People's Hospital),Jinan University / the First Affiliated Hospital (Shenzhen People's Hospital),Southern University of Science and Technology,Shenzhen,China
5.Forensics Genomics International (FGI),BGI-Shenzhen,Shenzhen,China
6.Department of Pulmonary and Critical Care Medicine,The First Affiliated Hospital of Guangxi Medical University,Nanning,China
7.Shenzhen Third People's Hospital,National Clinical Research Center for Infectious Disease,The Second Affiliated Hospital of Southern University of Science and Technology,Shenzhen,518112,China

Sun，Xiao Yu,Deng，Jie,Zhang，Chenhui,et al. Superoxide dismutase A (SodA) is a c-di-GMP effector protein governing oxidative stress tolerance in Stenotrophomonas maltophilia[J]. Microbiological Research,2024,278.

Sun，Xiao Yu.,Deng，Jie.,Zhang，Chenhui.,Fung，Sin Yee.,Siu，Kam Leung.,...&Yang，Liang.(2024).Superoxide dismutase A (SodA) is a c-di-GMP effector protein governing oxidative stress tolerance in Stenotrophomonas maltophilia.Microbiological Research,278.

Sun，Xiao Yu,et al."Superoxide dismutase A (SodA) is a c-di-GMP effector protein governing oxidative stress tolerance in Stenotrophomonas maltophilia".Microbiological Research 278(2024).