Biomechanical analysis of the bones in a rat model of osteoporosis based on the combination of disease and syndrome

Lin，Chu Bin1; He，Xing Peng2; Qiu，Yu Hui1; Wu，Wen Jin1; Chang，Yu1; Ye，Tao3; Li，Peng Fei4,5; Yang，Jian1

2024

10.12307/2024.420

Chinese Journal of Tissue Engineering Research   影响因子和分区

2095-4344

BACKGROUND: Kidney deficiency is the main pathogenesis of osteoporosis. To study the relationship between the two major syndrome types of kidney deficiency, Kidney-Yang deficiency and Kidney-Yin deficiency, is beneficial for the development of clinical diagnosis and treatments based on the combination of disease and syndrome. OBJECTIVE: To evaluate the biomechanical differences of the rat femurs with Kidney-Yang deficiency and Kidney-Yin deficiency caused by Yougui pills, and to demonstrate the scientific efficacy of medication based on the combination of disease and syndrome in osteoporosis from a biomechanical perspective. METHODS: The bilateral ovaries of 60 female Sprague-Dawley rats were surgically removed to establish an ovariectomized osteoporosis model. At 10 weeks after modeling, all the rats were randomly divided into a Kidney-Yang deficiency group (n=30) and a Kidney-Yin deficiency group (n=30). Rats with Kidney-Yang deficiency were given gluteal intramuscular injection of hydrocortisone, while rats with Kidney-Yin deficiency were orally administered with thyroid tablet suspension, once a day, for 14 consecutive days. After successful modeling, 20 rats in each group were given a suspension of Yougui pills by gavage once a day for 12 consecutive weeks and the remaining 10 rats were used as the control group without intervention. After gavage, the microstructural parameters of the bone were measured using Micro-CT scanning. Three-point bending, finite element simulation, femoral head compression, and surface indentation distribution experiments of the femurs were performed on a mechanical testing machine. RESULTS AND CONCLUSION: Micro-CT revealed that the femoral bone density, bone volume fraction, bone surface density, trabecular number, and trabecular separation were improved in the Kidney-Yin deficiency+Yougui pills group compared with the Kidney-Yin deficiency group (P < 0.05); the femoral bone volume fraction, bone surface density, trabecular number, and trabecular thickness were improved in the Kidney-Yang deficiency+Yougui pills group compared with the Kidney-Yang deficiency group (P < 0.05). The three-point bending experiment showed that the femur elastic modulus, maximum bending strength and bending fracture strength were decreased (P < 0.05) and toughness was increased (P < 0.05) in the Kidney-Yang deficiency+Yougui pills group compared with the Kidney-Yang deficiency group. Finite element simulation showed that Yougui pills could significantly improve the bending resistance of the femurs in the Kidney-Yang deficiency group, but had no significant effect on the Kidney-Yin deficiency group. The femoral head compression experiments showed that Yougui pills could enhance the ability of the femoral head to resist deformation in the Kidney-Yang deficiency group, but there was no significant difference in the effect of Yougui pills on the surface properties of the femoral head in the Kidney-Yin deficiency group and the Kidney-Yang deficiency group. To conclude, Yougui pills can significantly enhance the biomechanical properties of the osteoporotic bones with Kidney-Yang deficiency, but have no significant effect on the osteoporotic bone with Kidney-Yin deficiency.

biomechanics Kidney-Yang deficiency Kidney-Yin deficiency osteoporosis the combination of disease and syndrome Yougui pills

英语

其他

2-s2.0-85172307119

Scopus

1.Faculty of Intelligent Manufacturing,Wuyi University,Jiangmen,Guangdong Province,529020,China
2.Guangzhou University of Chinese Medicine,Guangzhou,Guangdong Province,510006,China
3.Department of Electrical and Electronics Engineering,Southern University of Science and Technology,Shenzhen,Guangdong Province,518055,China
4.Affiliated Jiangmen Hospital of Traditional Chinese Medicine,Jinan University,Jiangmen,Guangdong Province,529000,China
5.Jiangmen Central Hospital,Jiangmen,Guangdong Province,529020,China

Lin，Chu Bin,He，Xing Peng,Qiu，Yu Hui,et al. Biomechanical analysis of the bones in a rat model of osteoporosis based on the combination of disease and syndrome[J]. Chinese Journal of Tissue Engineering Research,2024,28(23):3636-3641.

Lin，Chu Bin.,He，Xing Peng.,Qiu，Yu Hui.,Wu，Wen Jin.,Chang，Yu.,...&Yang，Jian.(2024).Biomechanical analysis of the bones in a rat model of osteoporosis based on the combination of disease and syndrome.Chinese Journal of Tissue Engineering Research,28(23),3636-3641.

Lin，Chu Bin,et al."Biomechanical analysis of the bones in a rat model of osteoporosis based on the combination of disease and syndrome".Chinese Journal of Tissue Engineering Research 28.23(2024):3636-3641.