The application of quantitative telomerase activity measurement as an important indicator to monitor the cardiomyocyte differentiation process of human induced pluripotent stem cells under defined conditions

Ding，Shaoli1,2; Zhang，Rongzhi3; Zhang，Pengxia4; Shi，Jiamin5; Liu，Lu3; Li，Jiamin4; Zhang，Rui4; Wu，Fujian6; Zhou，Ping4

2023-12-20

10.1016/j.bbrc.2023.149150

Biochemical and Biophysical Research Communications   影响因子和分区

0006-291X

1090-2104

The construction of an in vitro differentiation system for human induced pluripotent stem cells (hiPSCs) has made exciting progress, but it is still of great significance to clarify the differentiation process. The use of conventional genetic and protein-labeled microscopes to observe or detect different stages of hiPSC differentiation is not specific enough and is cumbersome and time-consuming. In this study, in addition to analyzing the expression of gene/protein-related markers, we used a previously reported simple and excellent quantitative method of cellular telomerase activity based on a quartz crystal microbalance (TREAQ) device to monitor the dynamic changes in cellular telomerase activity in hiPSCs during myocardial differentiation under chemically defined conditions. Finally, by integrating these results, we analyzed the relationship between telomerase activity and the expression of marker genes/proteins as well as the cell type at each study time point. This dynamic quantitative measurement of cellular telomerase activity should be a promising indicator for monitoring dynamic changes in a stage of hiPSC differentiation and inducing cell types. This study provided a quantitative, dynamic and simple monitoring index for the in vitro differentiation process of hiPSC-CMs, which was a certain reference value for the optimization and improvement of the induction system.

Cell type Human induced pluripotent stem cells Myocardial differentiation Pluripotency Telomerase activity

SCI

英语

其他

WOS:001112809700001

BIOLOGY & BIOCHEMISTRY

2-s2.0-85175645278

Scopus

1.Department of Pain Treatment,Gansu Provincial Hospital,Lanzhou,China
2.The First School of Clinical Medicine,Lanzhou University,Lanzhou,China
3.Lanzhou University Second Hospital,Lanzhou University,Lanzhou,China
4.School of Stomatology,Lanzhou University,Lanzhou,No.222 Tianshui South Road, Chengguan District,730000,China
5.School of Life Sciences,Lanzhou University,Lanzhou,China
6.Translational Medicine Collaborative Innovation Center,Shenzhen People's Hospital (The First Affiliated Hospital,Southern University of Science and Technology,The Second Clinical Medical College of Jinan University),Shenzhen,Guangdong,518055,China

Ding，Shaoli,Zhang，Rongzhi,Zhang，Pengxia,et al. The application of quantitative telomerase activity measurement as an important indicator to monitor the cardiomyocyte differentiation process of human induced pluripotent stem cells under defined conditions[J]. Biochemical and Biophysical Research Communications,2023,687.

Ding，Shaoli.,Zhang，Rongzhi.,Zhang，Pengxia.,Shi，Jiamin.,Liu，Lu.,...&Zhou，Ping.(2023).The application of quantitative telomerase activity measurement as an important indicator to monitor the cardiomyocyte differentiation process of human induced pluripotent stem cells under defined conditions.Biochemical and Biophysical Research Communications,687.

Ding，Shaoli,et al."The application of quantitative telomerase activity measurement as an important indicator to monitor the cardiomyocyte differentiation process of human induced pluripotent stem cells under defined conditions".Biochemical and Biophysical Research Communications 687(2023).