Zymograph profiling reveals a divergent evolution of sirtuin that may originate from class III enzymes

Yang，Yujiao1,2,3; Zou，Siwei2; Cai，Kezhu2,4; Li，Ningning2; Li，Zhongyue2; Tan，Wei2; Lin，Wei5; Zhao，Guo Ping1,2,3; Zhao，Wei2

2023-11-01

10.1016/j.jbc.2023.105339

Journal of Biological Chemistry   影响因子和分区

0021-9258

1083-351X

Sirtuins are a group of NAD-dependent deacylases that conserved in three domains of life and comprehensively involved in the regulation of gene transcription, chromosome segregation, RNA splicing, apoptosis, and aging. Previous studies in mammalian cells have revealed that sirtuins not only exist as multiple copies, but also show distinct deacylase activities in addition to deacetylation. However, the understanding of sirtuin zymographs in other organisms with respect to molecular evolution remains at an early stage. Here, we systematically analyze the sirtuin activities in representative species from archaea, bacteria, and eukaryotes, using both the HPLC assay and a 7-amino-4-methylcoumarin-based fluorogenic method. Global profiling suggests that the deacylase activities of sirtuins could be divided into three categories and reveals undifferentiated zymographs of class III sirtuins, especially for those from bacteria and archaea. Nevertheless, initial differentiation of enzymatic activity was also observed for the class III sirtuins at both paralog and ortholog levels. Further phylogenetic analyses support a divergent evolution of sirtuin that may originate from class III sirtuins. Together, this work demonstrates a comprehensive panorama of sirtuin zymographs and provides new insights into the cellular specific regulation and molecular evolution of sirtuins.

activity profiling deacylase molecular evolution sirtuins zymograph

SCI ; EI

英语

NI论文

其他

Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB0480000] ; National Key R&D Program of China["2018YFA0903700","2019YFA0904003"] ; National Natural Science Foundation of China["319210026","U22A20540"] ; Guangdong Basic and Applied Basic Research Foundation[2023A1515030069]

Biochemistry & Molecular Biology

Biochemistry & Molecular Biology

WOS:001105399400001

ELSEVIER

20234515009797

Acetylation ; Cell death ; Mammals ; Molecular biology ; Transcription

Biology:461.9 ; Chemical Reactions:802.2

BIOLOGY & BIOCHEMISTRY

2-s2.0-85175424194

Scopus

1.CAS Key Laboratory of Synthetic Biology,CAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai,China
2.CAS Key Laboratory of Quantitative Engineering Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,China
3.University of Chinese Academy of Sciences,Beijing,China
4.Department of Materials Science and Engineering,School of Engineering,Southern University of Science and Technology,Shenzhen,China
5.Department of Pathogen Biology,School of Medicine & Holistic Integrative Medicine,Nanjing University of Chinese Medicine,Nanjing,China

Yang，Yujiao,Zou，Siwei,Cai，Kezhu,et al. Zymograph profiling reveals a divergent evolution of sirtuin that may originate from class III enzymes[J]. Journal of Biological Chemistry,2023,299(11).

Yang，Yujiao.,Zou，Siwei.,Cai，Kezhu.,Li，Ningning.,Li，Zhongyue.,...&Zhao，Wei.(2023).Zymograph profiling reveals a divergent evolution of sirtuin that may originate from class III enzymes.Journal of Biological Chemistry,299(11).

Yang，Yujiao,et al."Zymograph profiling reveals a divergent evolution of sirtuin that may originate from class III enzymes".Journal of Biological Chemistry 299.11(2023).