Single-cell communication patterns and their intracellular information flow in synovial fibroblastic osteoarthritis and rheumatoid arthritis

Wang，Jiajian1,2,3,4; Liu，Caihong5,6; Wang，Tingting7,8; Li，Sidi9; Bai，Yunmeng10; Pan，Fulin11; Wang，Jiayi12,13,14; Han，Jing15; Luo，Ruibin16; Wan，Xing1; Cui，Haiyan1; Huang，Yingcai1; Zheng，Mingqi1; Hong，Xiaoping7,17; Zhang，Jian V.2,3,4; Xu，Ruihuan1

2023-11-01

10.1016/j.imlet.2023.09.005

Immunology Letters   影响因子和分区

0165-2478

1879-0542

Background: Synovial fibroblasts are critical for maintaining homeostasis in major autoimmune diseases involving joint inflammation, including osteoarthritis and rheumatoid arthritis. However, little is known about the interactions among different cell subtypes and the specific sets of signaling pathways and activities that they trigger. Methods: Using social network analysis, pattern recognition, and manifold learning approaches, we identified patterns of single-cell communication in OA (osteoarthritis) and RA (rheumatoid arthritis). Results: Our results suggest that OA and RA have distinct cellular communication patterns and signaling pathways. The LAMININ (Laminin) and COLLAGEN (Collagen) pathways predominate in osteoarthritis, while the EGF (Epidermal growth factor), NT (Neurotrophin) and CDH5 (Cadherin 5) pathways predominate in rheumatoid arthritis, with a central role for THY1 (Thy-1 cell surface antigen) CDH11 (Cadherin 11) cells. The OA opens the PDGF (Platelet-derived growth factors) pathway (driver of bone angiogenesis), the RA opens the EGF pathway (bone formation) and the SEMA3 (Semaphorin 3A) pathway (involved in immune regulation). Interestingly, we found that OA no longer has cell types involved in the MHC complex (Major histocompatibility complex) and their activity, whereas the MHC complex functions primarily in RA in the presentation of inflammatory antigens, and that the complement system in OA has the potential to displace the function of the MHC complex. The specific signaling patterns of THY1CDH11 cells and their secreted ligand receptors are more conducive to cell migration and lay the foundation for promoting osteoclastogenesis. This subpopulation may also be involved in the accumulation of lymphocytes, affecting the recruitment of immune cells. Members of the collagen family (COL1A1 (Collagen Type I Alpha 1 Chain), COL6A2 (Collagen Type VI Alpha 2 Chain) and COL6A1 (Collagen Type VI Alpha 1 Chain)) and transforming growth factor (TGFB3) maintain the extracellular matrix in osteoarthritis and mediate cell migration and adhesion in rheumatoid arthritis, including the PTN (Pleiotrophin) / THBS1 (Thrombospondin 1) interaction. Conclusion: Increased understanding of the interaction networks between synovial fibroblast subtypes, particularly the shared and unique cellular communication features between osteoarthritis and rheumatoid arthritis and their hub cells, should help inform the design of therapeutic agents for inflammatory joint disease.

Cell membrane ligand/receptors Cellular communication pattern Osteoarthritis Rheumatoid arthritis Signaling pathway activities Single cell transcriptome Synovial fibroblasts THY1+CDH11+ hub cells

SCI

英语

其他

WOS:001081508100001

IMMUNOLOGY

2-s2.0-85171389845

Scopus

1.Clinical Laboratory Department of The Second Affiliated Hospital,School of Medicine,The Chinese University of Hong Kong,Shenzhen & Longgang District People's Hospital of Shenzhen,Shenzhen,518172,China
2.Center for Energy Metabolism and Reproduction,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China
3.Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China
4.Shenzhen Key Laboratory of Metabolic Health,Shenzhen,518055,China
5.School of Medicine,The Chinese University of Hong Kong,Shenzhen,518172,China
6.Biotechnology and Food Engineering Program,Guangdong Technion - Israel Institute of Technology,Shantou,515063,China
7.Department of Rheumatology and Immunology,The Second Clinical Medical College,Jinan University (Shenzhen People's Hospital),Shenzhen,518020,China
8.Integrated Chinese and Western Medicine Postdoctoral Research Station,Jinan University,Guangzhou,510632,China
9.Institute of Pathology and Southwest Cancer Center,Southwest Hospital,Army Medical University (Third Military Medical University),Chongqing,400038,China
10.Department of Nephrology,Shenzhen key Laboratory of Kidney Diseases,Shenzhen People's Hospital,the First Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
11.Rheumatology and Nephrology Department of The Second Affiliated Hospital,School of Medicine,The Chinese University of Hong Kong,Shenzhen & Longgang District People's Hospital of Shenzhen,Shenzhen,518172,China
12.First Affiliated Hospital of Anhui Medical university,Hefei,230022,China
13.First School of Clinical Medicine,Anhui Medical University,Hefei,230032,China
14.School of Basic Medical Sciences,Anhui Medical University,Hefei,230032,China
15.Warshel Institute for Computational Biology,School of Life and Health Sciences,The Chinese University of Hong Kong,Shenzhen,Shenzhen,518172,China
16.Department of Clinical Laboratory,Longgang District Central Hospital of Shenzhen,Shenzhen,Guangdong,518116,China
17.Department of Rheumatology and Immunology,The Frist Affiliated Hospital (Shenzhen People's Hospital),Southern University of Science and Technology,Shenzhen,518055,China

Wang，Jiajian,Liu，Caihong,Wang，Tingting,et al. Single-cell communication patterns and their intracellular information flow in synovial fibroblastic osteoarthritis and rheumatoid arthritis[J]. Immunology Letters,2023,263:1-13.

Wang，Jiajian.,Liu，Caihong.,Wang，Tingting.,Li，Sidi.,Bai，Yunmeng.,...&Xu，Ruihuan.(2023).Single-cell communication patterns and their intracellular information flow in synovial fibroblastic osteoarthritis and rheumatoid arthritis.Immunology Letters,263,1-13.

Wang，Jiajian,et al."Single-cell communication patterns and their intracellular information flow in synovial fibroblastic osteoarthritis and rheumatoid arthritis".Immunology Letters 263(2023):1-13.