Microevolution of Salmonella 4,[5],12:i:- derived from Salmonella enterica serovar Typhimurium through complicated transpositions

Wang，Zhenyu1,2,3; Gu，Dan1,2,3; Hong，Yaming1,2,3; Hu，Yachen1,2,3; Gu，Jiaojie1,2,3; Tang，Yuanyue1,2,3; Zhou，Xiaohui4,5; Zhang，Yunzeng1,2,3; Jiao，Xinan1,2,3; Li，Qiuchun1,2,3

2023-10-31

10.1016/j.celrep.2023.113227

Cell Reports   影响因子和分区

2211-1247

2211-1247

Salmonella enterica subsp. enterica serovar 4,[5],12:i:- (Salmonella 4,[5],12:i:-), derived from S. Typhimurium, has become the dominant serotype causing human salmonellosis. In this study, we define the genetic mechanism of the generation of Salmonella 4,[5],12:i:- from S. Typhimurium through complicated transpositions and demonstrate that Salmonella 4,[5],12:i:- displays more efficient colonization and survival abilities in mice than its parent S. Typhimurium strain. We identified intermediate strains carrying both resistance regions (RRs) and the fljAB operon for the generation of Salmonella 4,[5],12:i:-. The insertion of RR3 into the chromosomal hin-iroB site of S. Typhimurium produced RR3-S. Typhimurium as a primary intermediate. Salmonella 4,[5],12:i:- was then produced by replacing the fljAB operon and/or its flanking sequences through intramolecular transpositions mediated by IS26 and/or IS1R elements in RR3-S. Typhimurium, which was further confirmed both in vitro and in vivo. Overall, we demonstrate the molecular mechanism underlying the origin, generation, and advantage of RRs-Salmonella 4,[5],12:i:- from S. Typhimurium.

CP: Microbiology IS1R IS26 resistance regions (RRs) Salmonella enterica serovar Typhimurium (S. Typhimurium) Salmonella enterica subsp. enterica serovar 4,[5],12:i:- (Salmonella 4,[5],12:i:-)

SCI

英语

其他

National Key Research and Development Program of China[2022YFC2604200] ; National Natural Science Foundation of China["31920103015","32072821"] ; fifth phase of the "333 project"scientific research project in Jiangsu Province[BRA2020002] ; Postgraduate Research & Practice Innovation Program of Jiangsu Province (Yangzhou University)[KYCX22_3536]

Cell Biology

Cell Biology

WOS:001096475700001

CELL PRESS

2-s2.0-85173921113

Scopus

1.Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-food Safety and Quality,Ministry of Agriculture of China,Yangzhou University,Yangzhou,48 Wenhui East Road, Jiangsu,225000,China
2.Jiangsu Key Lab of Zoonosis/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Yangzhou,48 Wenhui East Road, Jiangsu,225000,China
3.Joint International Research Laboratory of Agriculture and Agri-product Safety,Yangzhou University,Yangzhou,48 Wenhui East Road, Jiangsu,225000,China
4.Department of Pathobiology and Veterinary Science,University of Connecticut,Storrs,06269,United States
5.School of Public Health and Emergency Management,Southern University of Science and Technology,Shenzhen,1088 Xueyuan Road, Nanshan District, Guangdong,518055,China

Wang，Zhenyu,Gu，Dan,Hong，Yaming,et al. Microevolution of Salmonella 4,[5],12:i:- derived from Salmonella enterica serovar Typhimurium through complicated transpositions[J]. Cell Reports,2023,42(10).

Wang，Zhenyu.,Gu，Dan.,Hong，Yaming.,Hu，Yachen.,Gu，Jiaojie.,...&Li，Qiuchun.(2023).Microevolution of Salmonella 4,[5],12:i:- derived from Salmonella enterica serovar Typhimurium through complicated transpositions.Cell Reports,42(10).

Wang，Zhenyu,et al."Microevolution of Salmonella 4,[5],12:i:- derived from Salmonella enterica serovar Typhimurium through complicated transpositions".Cell Reports 42.10(2023).