| 题名 | A phenotype-directed chemical screen identifies ponalrestat as an inhibitor of the plant flavin monooxygenase YUCCA in auxin biosynthesis |
| 作者 | |
| 通讯作者 | Jiang，Kai; Guo，Hongwei |
| 发表日期 | 2019
|
| DOI | |
| 发表期刊 | |
| ISSN | 0021-9258
|
| EISSN | 1083-351X
|
| 卷号 | 294期号:52页码:19923-19933 |
| 摘要 | Plant development is regulated by both synergistic and antagonistic interactions of different phytohormones, including a complex crosstalk between ethylene and auxin. For instance, auxin and ethylene synergistically control primary root elongation and root hair formation. However, a lack of chemical agents that specifically modulate ethylene or auxin production has precluded precise delineation of the contribution of each hormone to root development. Here, we performed a chemical genetic screen based on the recovery of root growth in ethylene-related Arabidopsis mutants with constitutive "short root" phenotypes (eto1-2 and ctr1-1).Wefound that ponalrestat exposure recovers root elongation in these mutants in an ethylene signal-independent manner. Genetic and pharmacological investigations revealed that ponalrestat inhibits the enzymatic activity of the flavin-containing monooxygenase YUCCA, which catalyzes the rate-limiting step of the indole-3-pyruvic acid branch of the auxin biosynthesis pathway. In summary, our findings have identified a YUCCA inhibitor that may be useful as a chemical tool to dissect the distinct steps in auxin biosynthesis and in the regulation of root development. |
| 关键词 | |
| 相关链接 | [Scopus记录] |
| 收录类别 | |
| 语种 | 英语
|
| 学校署名 | 第一
; 通讯
|
| 资助项目 | Shenzhen Sci-Tech Fund[KYTDPT20181011104005]
|
| WOS研究方向 | Biochemistry & Molecular Biology
|
| WOS类目 | Biochemistry & Molecular Biology
|
| WOS记录号 | WOS:000505005800009
|
| 出版者 | |
| EI入藏号 | 20200207993449
|
| EI主题词 | Biochemistry
; Biosynthesis
|
| EI分类号 | Biochemistry:801.2
; Organic Compounds:804.1
|
| ESI学科分类 | BIOLOGY & BIOCHEMISTRY
|
| Scopus记录号 | 2-s2.0-85077295466
|
| 来源库 | Scopus
|
| 引用统计 |
被引频次[WOS]：15
|
| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/60904 |
| 专题 | 南方科技大学-北京大学植物与食品联合研究所 生命科学学院 前沿与交叉科学研究院 生命科学学院_生物系 |
| 作者单位 | 1.Department of Biology,Institute of Plant and Food Science,Southern University of Science and Technology (SUSTech),Shenzhen, Guangdong,518055,China 2.State Key Laboratory of Protein and Plant Gene Research,College of Life Science,Peking University,Beijing,100871,China 3.Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education and Beijing National Laboratory for Molecular Science (BNLMS) and Peking-Tsinghua Center for Life Sciences,Peking University,Beijing,100871,China 4.Max-Planck Institute for Plant Breeding Research,Cologne,50829,Germany 5.Plant Molecular and Cellular Biology Laboratory,Salk Institute for Biological Studies,San Diego,92037,United States 6.Southern University of Science and Technology (SUSTech),SUSTech Academy for Advanced and Interdisciplinary Studies,Shenzhen, Guangdong,518055,China 7.BioCentury Inc,Redwood City,94065,United States 8.Dept. of Chemistry,Johns Hopkins University,Baltimore,21218,United States 9.School of Pharmacy,North Sichuan Medicine College,Nanchong,637100,China 10.Dept. of Pathology,Johns Hopkins University School of Medicine,Baltimore,21231,United States 11.State Key Laboratory of Chemical Oncogenomics and Key Laboratory of Chemical Genomics,Peking University Shenzhen Graduate School,Shenzhen,518055,China |
| 第一作者单位 | 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 通讯作者单位 | 南方科技大学; 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 第一作者的第一单位 | 南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院 |
| 推荐引用方式 GB/T 7714 |
Zhu，Ying,Li，Hong Jiang,Su，Qi,et al. A phenotype-directed chemical screen identifies ponalrestat as an inhibitor of the plant flavin monooxygenase YUCCA in auxin biosynthesis[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2019,294(52):19923-19933.
|
| APA |
Zhu，Ying.,Li，Hong Jiang.,Su，Qi.,Wen，Jing.,Wang，Yuefan.,...&Guo，Hongwei.(2019).A phenotype-directed chemical screen identifies ponalrestat as an inhibitor of the plant flavin monooxygenase YUCCA in auxin biosynthesis.JOURNAL OF BIOLOGICAL CHEMISTRY,294(52),19923-19933.
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| MLA |
Zhu，Ying,et al."A phenotype-directed chemical screen identifies ponalrestat as an inhibitor of the plant flavin monooxygenase YUCCA in auxin biosynthesis".JOURNAL OF BIOLOGICAL CHEMISTRY 294.52(2019):19923-19933.
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| 条目包含的文件 | 条目无相关文件。 | |||||
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