A Subunit Vaccine Candidate Composed of Mpox Virus A29L, M1R, A35R, and B6R Elicits Robust Immune Response in Mice

Yang, Xuetao1,2; Yang, Xidan1,2; Du, Shouwen3; Hu, Congxia1; Yang, Xiu1,2; Wang, Xingyun4; Hu, Xing4; Rcheulishvili, Nino4,5; Wang, Peng George2,4; Lin, Jihui1,2,3

2023-09-01

10.3390/vaccines11091420

VACCINES   影响因子和分区

2076-393X

With no specific antiviral drugs and preventive vaccines against Mpox virus (MPXV), the epidemic has led to the declaration of a Public Health Emergency of International Concern. As a developmental direction for new vaccines, studies of subunit vaccines based upon MPXV antigen proteins are lacking. In this study, A29L, M1R, A35R, and B6R of MPXV were expressed and purified from a prokaryotic system. The four MPXV antigen proteins in combination were mixed with aluminum hydroxide or CpG7909 as adjuvant, and subsequently used to inoculate mice. The results of enzyme-linked immunosorbent assay (ELISA), flow cytometry analyses, and enzyme-linked immunospot (ELISPOT) assays indicated that A29L, M1R, A35R, and B6R elicited high-level antigen-specific antibodies and CD4+ T cells-based cellular immune response in mice. Moreover, the results of virus neutralization assays suggested that sera from the mice immunized with four proteins elicited high neutralizing activities against the vaccinia virus. Notably, the results of ELISA, ELISPOT, and virus neutralization assays also showed that the CpG7909 adjuvant was more effective in inducing an immune response compared with the aluminum adjuvant. In summary, this study offers valuable insights for further studies of subunit vaccine candidates for the prevention of MPXV and other orthomyxoviruses.

Mpox virus subunit vaccine CpG7909 adjuvant prokaryotic expression immune response

SCI

英语

通讯

Postdoctoral Science Foundation of China[2020T130044ZX] ; Shenzhen Science and Technology Innovation Commission[KQTD20200909113758004] ; Shenzhen Key Laboratory of Prevention and the Treatment of Severe Infections[ZDSYS20200811142804014] ; Shenzhen Key Medical Discipline Construction Fund[SZXK045]

Immunology ; Research & Experimental Medicine

Immunology ; Medicine, Research & Experimental

WOS:001078801400001

MDPI

Web of Science

1.Southwest Med Univ, Sch Nursing, Luzhou 646000, Peoples R China
2.Pengbo Biotechnol Co Ltd, Shenzhen 518000, Peoples R China
3.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Infect Dis, Shenzhen 518020, Peoples R China
4.Southern Univ Sci & Technol, Sch Med, Dept Pharmacol, Shenzhen 518000, Peoples R China
5.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China

Yang, Xuetao,Yang, Xidan,Du, Shouwen,et al. A Subunit Vaccine Candidate Composed of Mpox Virus A29L, M1R, A35R, and B6R Elicits Robust Immune Response in Mice[J]. VACCINES,2023,11(9).

Yang, Xuetao.,Yang, Xidan.,Du, Shouwen.,Hu, Congxia.,Yang, Xiu.,...&Lin, Jihui.(2023).A Subunit Vaccine Candidate Composed of Mpox Virus A29L, M1R, A35R, and B6R Elicits Robust Immune Response in Mice.VACCINES,11(9).

Yang, Xuetao,et al."A Subunit Vaccine Candidate Composed of Mpox Virus A29L, M1R, A35R, and B6R Elicits Robust Immune Response in Mice".VACCINES 11.9(2023).